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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Nandrolone decanoate negatively reverses the beneficial effects of exercise on cardiac muscle via sarcolemmal, but not mitochondrial K-ATP channel
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Nandrolone decanoate negatively reverses the beneficial effects of exercise on cardiac muscle via sarcolemmal, but not mitochondrial K-ATP channel

机译：Nandrolone Decanoate负面逆转通过Sarcolemmal锻炼对心肌的有益效果，但不是线粒体K-ATP通道

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摘要

ATP-sensitive potassium channels are supposed to have a substantial role in improvement of cardiac performance. This study was performed to evaluate whether nandrolone decanoate (ND) and (or) exercise training could affect the expression of cardiac K-ATP channel subunits. Thirty-five male albino Wistar rats were randomly divided into 5 groups, including sedentary control (SC), sedentary vehicle (SV), sedentary ND (SND), exercise control (EC), and exercise and ND (E+ND). Exercise training was performed on a treadmill 5 times per week. ND was injected (10 mg/kg/week, i.m.) to the rats in the SND and E+ND groups. Following cardiac isolation, the expression of both sarcolemmal and mitochondrial subunits of K-ATP channel was measured using Western blot method. The expression of sarcolemmal, but not mitochondrial, subunits of K-ATP channel (Kir(6.2) and SUR2) of EC group was significantly higher compared with SC group while ND administration (SND group) did not show any change in their expression. In the E+ND group, ND administration led to decrease of the over-expression of sarcolemmal Kir(6.2) and SUR2 which was previously induced by exercise. There was no significant association between the mitochondrial expression of either Kir(6.2) or SUR2 proteins and administration of ND or exercise. Supra-physiological dosage of ND negatively reverses the effects of exercise on the cardiac muscle expression of sarcolemmal, but not mitochondrial, K-ATP channel subunits.
机译：ATP敏感的钾通道应该在改善心脏能方面具有重要作用。进行该研究以评估Nandrolone癸酸酯（Nd）和（或）运动训练是否可能影响心脏K-ATP频道亚基的表达。将三十五只雄性白化Wistar大鼠随机分为5组，包括久坐不动（SC），久坐型载体（SV），久坐不动ND（SND），运动控制（EC）和运动，ND（E + Nd）。运动训练每周5次跑步机进行。将ND注入（10mg / kg /周，即）到SND和E + ND组中的大鼠。在心脏隔离后，使用蛋白质印迹法测量K-ATP通道的SARCOMMAL和线粒体亚基的表达。与SC组相比，菌本肠道（KIR（6.2）和SUR2）的K-ATP通道（KIR（6.2）和SUR2）的表达，EC组的表达明显高于，而ND施用（SND组）没有显示出表达的任何变化。在E + ND组中，ND管理导致患Sarcolemmal KIR（6.2）和SUR2的过度表达，其先前通过运动诱导。 KIR（6.2）或SUR2蛋白的线粒体表达与ND或运动施用之间没有显着关联。 ND的Supra生理剂量负面逆转运动对Sarcolemmal的心肌表达的影响，但不是线粒体，K-ATP频道亚基。

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