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首页> 外文期刊>Cytometry: The Journal of the Society for Analytical Cytology >Immunophenotypic characterization of myelopoiesis in early and late myelodysplastic syndromes: Use of CD44 as an aid in early diagnosis
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Immunophenotypic characterization of myelopoiesis in early and late myelodysplastic syndromes: Use of CD44 as an aid in early diagnosis

机译：早期和晚期骨髓增生异常综合症的骨髓生成的免疫表型特征：CD44辅助早期诊断

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摘要

We investigated the possibility that myeloid cells from the bone marrow (BM) of myelodysplastic patients differ in their expression of CD44 antigen compared with expression of the antigen in normal controls. In addition, two triple-surface marker assays incorporating, respectively, CD44/CD33/CD66 and CD33/CD34/ HLA-DR were used to evaluate the degree of myeloid maturation and assess the number of blasts in BM by flow cytometry. Patients with early-stage myelodysplastic syndrome (MDS; RA [FAB classification]) have significantly decreased expression of CD44 on gated myeloid cells. In contrast, patients with late-stage MDS (RAEB and RAEB-T [FAB classification]) showed an elevated expression of CD44 and an increased number of CD34 blasts compared with early-stage MDS patients and normal controls. Late-stage MDS patients also had an increase in the immature myeloid compartment (CD66 weak expression) compared with early-stage MDS patients and normal controls. We have already included this assay as part of our MDS evaluation protocol alongside BM morphology and cytogenetics. (C) 2002 Wiley-Liss, Inc. [References: 32]
机译：我们调查了骨髓增生异常患者骨髓（BM）的髓样细胞与正常对照中抗原表达相比CD44抗原表达不同的可能性。另外，分别结合了CD44 / CD33 / CD66和CD33 / CD34 / HLA-DR的两种三重表面标记测定法被用于评估骨髓成熟程度并通过流式细胞术评估BM中的胚泡数目。患有早期骨髓增生异常综合症（MDS; RA [FAB分类]）的患者在门控髓样细胞上CD44的表达明显降低。相反，与早期MDS患者和正常对照相比，晚期MDS患者（RAEB和RAEB-T [FAB分类]）显示CD44表达升高，CD34胚细胞数量增加。与早期MDS患者和正常对照组相比，晚期MDS患者的未成熟骨髓腔（CD66弱表达）也有所增加。我们已经将该检测方法与BM形态学和细胞遗传学一起纳入了MDS评估方案。 （C）2002 Wiley-Liss，Inc. [参考：32]

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