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首页> 外文期刊>Canadian Journal of Plant Science >A second set of XEGIP-encoding genes resides on chromosome 8 of potato and tomato
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A second set of XEGIP-encoding genes resides on chromosome 8 of potato and tomato

机译:第二组XegIP编码基因位于马铃薯和番茄的染色体上

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摘要

Xyloglucan-specific endoglucanase inhibitor proteins (XEGIPs) are present in a wide range of dicots, where they are believed to play a role in defense from pathogens. XEGIPs are generally present as two or three copies; however, they are reported to be present as a cluster of 10 copies in potato and tomato on chromosome 1. We have identified a second set of XEGIP-encoding genes, where two inverted copies are present on chromosome 8 of potato (Solanum tuberosum L.). The same set exists in the same order on chromosome 8 of tomato (Solanum pennellii Correll). Transcript expression analysis indicates that XEGIP10 is transcribed only in roots, while XEGIP11 transcripts were not detected under normal growth conditions. Transformed potatoes ('Bintje' and 'Kennebec') overexpressing these genes did not show any changes in phenology. Foliar screening of transgenic lines for resistance to the late blight pathogen Phytophthora infestans (Mont.) de Bary did not demonstrate a large reduction in disease progression or pathogen sporulation.
机译:Xyloglucan特异性内葡聚糖酶酶抑制剂蛋白质(Xegips)存在于各种Dicots中,在那里他们被认为在病原体中发挥作用。 Xegips通常存在为两三个副本;然而,据报道,它们在染色体1上作为马铃薯和番茄中的10个拷贝的群体存在。我们已经确定了第二组XegIP编码基因,其中存在两组倒拷贝在马铃薯染色体8(Solanum Tuberosum L. )。在番茄(Solanum Pennellii Correll)的染色体8上的相同顺序中存在相同的组。转录表达分析表明XEGIP10仅在根中转录,而在正常生长条件下未检测到XEGIP11转录物。转化的土豆('bintje'和'kennebec')过表达这些基因没有显示出候选的任何变化。叶面筛选转基因患者抗性抗性病原体植物植物植物(MONT。)DE BARY未表明疾病进展或病原体孢子的大量减少。

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