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首页> 外文期刊>Cytokine >An extract based on the medicinal mushroom Agaricus blazei Murill stimulates monocyte-derived dendritic cells to cytokine and chemokine production in vitro.
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An extract based on the medicinal mushroom Agaricus blazei Murill stimulates monocyte-derived dendritic cells to cytokine and chemokine production in vitro.

机译：基于药用蘑菇姬松茸的提取物在体外刺激单核细胞衍生的树突状细胞产生细胞因子和趋化因子。

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摘要

The edible mushroom Agaricus blazei Murill (AbM), which has been used in traditional medicine against a range of diseases and possess immunomodulating properties, probably due to its high content of beta-glucans. Others and we have demonstrated stimulatory effects of extracts of this mushroom on different immune cells. Dendritic cells are major directors of immune function. We wanted to examine the effect of AbM stimulation on signal substance release from monocyte-derived dendritic cells (MDDC). After 6d incubation with IL-4 and GM-CSF, the cells were true MDDC. Then the cells were further incubated with up to 10% of the AbM-based extract, AndoSan, LPS (0.5 microg/ml) or PBS control. We found that the AbM extract promoted dose-dependent increased levels of IL-8, G-CSF, TNFalpha, IL-1beta, IL-6 and MIP-1beta, in that order. The synthesis of IL-2, IL-8 and IFNgamma were similar for the AbM extract and LPS. However, AndoSan induced a 10- to 2-fold higher production than did LPS of G-CSF, TNFalpha and IL-1beta, respectively. AbM did not induce increased synthesis of Th2 or anti-inflammatory cytokines or the Th1 cytokine IL-12. We conclude that stimulation of MDDC with an AbM-based extract resulted in increased production of proinflammatory, chemotactic and some Th1-type cytokines in vitro.
机译：食用蘑菇姬松茸（AbM）已用于多种疾病的传统药物中，并具有免疫调节特性，这可能是由于其高含量的β-葡聚糖。其他人和我们已经证明了该蘑菇提取物对不同免疫细胞的刺激作用。树突状细胞是免疫功能的主要指导者。我们想要研究AbM刺激对单核细胞衍生树突状细胞（MDDC）释放信号物质的影响。与IL-4和GM-CSF孵育6天后，细胞为真MDDC。然后将细胞与最多10％的基于AbM的提取物，AndoSan，LPS（0.5 microg / ml）或PBS对照一起孵育。我们发现AbM提取物按此顺序促进了剂量依赖性的IL-8，G-CSF，TNFalpha，IL-1beta，IL-6和MIP-1beta升高水平。对于AbM提取物和LPS，IL-2，IL-8和IFNγ的合成相似。但是，AndoSan诱导的产量分别比G-CSF，TNFα和IL-1beta的LPS高10到2倍。 AbM不会诱导Th2或抗炎细胞因子或Th1细胞因子IL-12的合成增加。我们得出结论，用基于AbM的提取物刺激MDDC导致体外促炎性，趋化性和某些Th1型细胞因子的产生增加。

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