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首页> 外文期刊>Cytokine >Modulation of human beta-defensin-2 expression by 17beta-estradiol and progesterone in vaginal epithelial cells.
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Modulation of human beta-defensin-2 expression by 17beta-estradiol and progesterone in vaginal epithelial cells.

机译：阴道上皮细胞中17beta-雌二醇和孕酮对人beta-defensin-2表达的调节。

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摘要

We investigated the expression of HBD-1 and -2 in vaginal epithelial cells treated with lipopolysaccharide (LPS) and the effects on HBD-2 expressions by 17beta-estradiol and progesterone. Primary vaginal epithelial cells were isolated from a segment of normal anterior vaginal wall obtained during vaginoplasty and were cultured in keratinocyte growth medium and were allowed to undergo their 3rd passage. Expression of HBD-1 and -2 by different stimuli using LPS 0.5 microg/ml, 17beta-estradiol 2 nM and progesterone 1 microM was measured by RT-PCR, ELISA and real-time RT-PCR, respectively. HBD-1 was produced constitutively in vaginal epithelial cells and the production of HBD-1 was not influenced by LPS, 17beta-estradiol and progesterone, but the production of HBD-2 was increased inducibly by LPS. 17beta-Estradiol and progesterone did not change the production of HBD-2 in normal state, but 17beta-estradiol increased the production of HBD-2 and progesterone suppressed the production of HBD-2 under the circumstances with infection. The HBD-2 plays an important role at innate host defense on genitourinary tract. The lacks of estrogen during menopause or uses of a progesterone-based oral contraceptive in sexually active women may influence production of HBD-2 in vaginal epithelium and may increase susceptibility to bacterial vaginitis or recurrent UTI.
机译：我们调查了脂多糖（LPS）处理的阴道上皮细胞中HBD-1和-2的表达以及17β-雌二醇和孕酮对HBD-2表达的影响。从在阴道成形术中获得的正常阴道前壁的一部分中分离出原代阴道上皮细胞，并在角质形成细胞生长培养基中进行培养，并使其进行第三次传代。通过RT-PCR，ELISA和实时RT-PCR分别测量了使用LPS 0.5 microg / ml，17beta-雌二醇2 nM和孕酮1 microM在不同刺激下HBD-1和-2的表达。 HBD-1在阴道上皮细胞中组成性地产生，而HBD-1的产生不受LPS，17β-雌二醇和孕酮的影响，但是HBD-2的产生可通过LPS诱导增加。在正常情况下，17β-雌二醇和孕酮不会改变HBD-2的产生，但是17β-雌二醇会增加HBD-2的产生，而孕酮会抑制HBD-2的产生。 HBD-2在泌尿生殖道的先天宿主防御中起着重要作用。更年期期间缺乏雌激素或性活跃妇女使用基于孕激素的口服避孕药可能会影响阴道上皮中HBD-2的产生，并可能增加对细菌性阴道炎或复发性UTI的敏感性。

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