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首页> 外文期刊>Cell biology international. >Human bone marrow‐derived MSCs spontaneously express specific Schwann cell markers
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Human bone marrow‐derived MSCs spontaneously express specific Schwann cell markers

机译：人骨髓衍生的MSCs自发地表达特定的施旺细胞标记

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Abstract In peripheral nerve injuries, Schwann cells (SC) play pivotal roles in regenerating damaged nerve. However, the use of SC in clinical cell‐based therapy is hampered due to its limited availability. In this study, we aim to evaluate the effectiveness of using an established induction protocol for human bone marrow derived‐MSC (hBM‐MSCs) transdifferentiation into a SC lineage. A relatively homogenous culture of hBM‐MSCs was first established after serial passaging (P3), with profiles conforming to the minimal criteria set by International Society for Cellular Therapy (ISCT). The cultures ( n ?=?3) were then subjected to a series of induction media containing β‐mercaptoethanol, retinoic acid, and growth factors. Quantitative RT‐PCR, flow cytometry, and immunocytochemistry analyses were performed to quantify the expression of specific SC markers, that is, S100, GFAP, MPZ and p75 NGFR, in both undifferentiated and transdifferentiated hBM‐MSCs. Based on these analyses, all markers were expressed in undifferentiated hBM‐MSCs and MPZ expression (mRNA transcripts) was consistently detected before and after transdifferentiation across all samples. There was upregulation at the transcript level of more than twofolds for NGF, MPB, GDNF, p75 NGFR post‐transdifferentiation. This study highlights the existence of spontaneous expression of specific SC markers in cultured hBM‐MSCs, inter‐donor variability and that MSC transdifferentiation is a heterogenous process. These findings strongly oppose the use of a single marker to indicate SC fate. The heterogenous nature of MSC may influence the efficiency of SC transdifferentiation protocols. Therefore, there is an urgent need to re‐define the MSC subpopulations and revise the minimal criteria for MSC identification.
机译：摘要在外周神经损伤中，施旺细胞（SC）在再生受损神经中起枢转作用。然而，由于其可用性有限，临床细胞疗法中的使用受到阻碍。在这项研究中，我们的目标是评估使用人骨髓衍生MSC（HBM-MSCs）转换为SC谱系的良好诱导方案的有效性。首先在连续传代（P3）之后第一次建立相对均匀的HBM-MSC培养物，曲线符合国际细胞疗法（ISCT）所设定的最小标准（ISCT）。然后对培养物（n？=β3）进行含有β-巯基乙醇，视黄酸和生长因子的一系列感应培养基。进行定量RT-PCR，流式细胞术和免疫细胞化学分析以量化特异性SC标记的表达，即S100，GFAP，MPZ和P75 NGFR，在未分化的和转置的HBM-MSC中。基于这些分析，所有标记物在未分化的HBM-MSCs中表达，并且在所有样品的转晶前后始终检测MPz表达（mRNA转录物）。对于NGF，MPB，GDNF，P75 NGFR后转移后异细胞素的转录物水平超过双重细胞。该研究突出显示培养的HBM-MSCs，供体间变异性的特异性SC标记的自发表达，并且MSC转置异化是异丙状过程。这些发现强烈反对使用单个标记来指示SC命运。 MSC的异质性质可能影响SC转晶类别的效率。因此，迫切需要重新定义MSC子步骤并修改MSC识别的最小标准。

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