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首页> 外文期刊>Cell biology international. >Effect of glucose and palmitate environment on proliferation and migration of PC3‐prostate cancer cells
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Effect of glucose and palmitate environment on proliferation and migration of PC3‐prostate cancer cells

机译：葡萄糖和棕榈酸盐环境对PC3-前列腺癌细胞增殖和迁移的影响

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Abstract Recent studies have been trying to find out how diet and metabolic changes such as dyslipidaemia, hyperglycaemia, and hyperinsulinaemia can stimulate cancer progression. This investigation aimed to evaluate the effect of high concentrations of fatty acids and/or glucose in tumour prostate cells, focusing on the proliferation/migration profile and oxidative stress. PC3 cells were treated with high concentration of saturated fatty acid (palmitate, 100?μM), glucose (220?mg/dL), or both for 24 or 48?h. Results demonstrated that PC3 cells showed a significant increase in proliferation after 48?h of treatment with glucose and palmitate+glucose. Cell proliferation was associated with reduced levels of AMPK phosphorylation in glucose group at 24 and 48?h of treatment, while palmitate group presented this result only after 48?h of treatment. Also, there was a significant increase in cell migration between time 0 and 48?h after all treatments, except in the control. Catalase activity was increased by palmitate in the beginning of treatment, while glucose presented a later effect. Also, nitrite production was increased by glucose only after 48?h, and the total antioxidant activity was enhanced by palmitate in the initial hours. Thus, we conclude that the high concentration of the saturated fatty acid palmitate and glucose in vitro influences PC3 cells and stimulates cellular activities related to carcinogenesis such as cell proliferation, migration, and oxidative stress in different ways. Palmitate presents a rapid and initial effect, while a glucose environment stimulates cells later on, maintaining high levels of cell proliferation.
机译：摘要最近的研究一直在努力了解饮食和代谢变化，如血脂血症，高血糖和高胰岛素血症可以刺激癌症进展。该研究旨在评估高浓度脂肪酸和/或葡萄糖在肿瘤前列腺细胞中的影响，重点是增殖/迁移曲线和氧化应激。用高浓度的饱和脂肪酸（棕榈酸盐，100μm），葡萄糖（220×mg / dL）或24或48℃处理PC3细胞。结果表明，用葡萄糖和棕榈酸盐+葡萄糖治疗48℃后，PC3细胞的增殖显着增加。细胞增殖与葡萄糖组中的AMPK磷酸化水平降低有关，在治疗中，棕榈酸盐组仅在48℃治疗后呈现出该结果。此外，除了控制之外，在所有治疗之后，时间0和48Ωh之间的细胞迁移显着增加。在治疗开始时，通过棕榈酸糖增加了过氧化氢酶活性，而葡萄糖呈现出后来的效果。此外，仅在48℃之后通过葡萄糖产生亚硝酸盐产量，并且在初始小时内通过棕榈酸棕榈酸盐增强总抗氧化活性。因此，我们得出结论，饱和脂肪酸棕榈酸盐和体外葡萄糖的高浓度影响PC3细胞并刺激与致癌物相关的细胞活性，例如细胞增殖，迁移和氧化应激。棕榈酸呈快速且初始效果，而葡萄糖环境促使细胞以后，保持高水平的细胞增殖。

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