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首页> 外文期刊>Cell biology international. >WDR13 promotes the differentiation of bovine skeletal muscle‐derived satellite cells by affecting PI3K/AKT signaling
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WDR13 promotes the differentiation of bovine skeletal muscle‐derived satellite cells by affecting PI3K/AKT signaling

机译：WDR13通过影响PI3K / AKT信号传导来促进牛骨骼肌衍生卫星细胞的分化

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摘要

Abstract Muscle satellite cells are usually at rest, and when externally stimulated or regulated, they can be further differentiated by cell fusion to form new myotubes and muscle fibers. WD repeat domain 13 (WDR13) is highly conserved in vertebrates. Studies have shown that mice lacking the Wdr13 gene develop mild obesity, hyperinsulinemia, and increased islet β cell proliferation. However, the role of WDR13 in bovine cells is unclear. Here, we investigated the effect of WDR13 on bovine skeletal muscle‐derived satellite cells (MDSCs). We found that WDR13 was upregulated in bovine MDSCs using western blotting and immunofluorescence experiments. Moreover, activation and inhibition of WDR13 expression increased and decreased cell differentiation, respectively, suggesting that WDR13 promotes bovine MDSC differentiation. To further understand the mechanism of action of WDR13, we examined changes in the PI3K/AKT signaling pathway following WDR13 activation or inhibition. In addition, cells were treated with a phosphoinositide kinase 3 (PI3K) inhibitor, LY294004, to observe cell differentiation. The results showed that activation of WDR13 inhibited the PI3K/AKT signaling pathway and enhanced cell differentiation. These data suggest that WDR13 can promote the differentiation of bovine MDSCs by affecting the PI3K/AKT signaling pathway.
机译：摘要肌肉卫星细胞通常在静止，并且在外部刺激或调节时，它们可以通过细胞融合进一步分化，形成新的肌管和肌肉纤维。 WD重复域13（WDR13）在脊椎动物中高度保守。研究表明，缺乏WDR13基因的小鼠产生轻度肥胖，高胰岛素血症和增加的胰岛β细胞增殖。然而，WDR13在牛细胞中的作用尚不清楚。在这里，我们研究了WDR13对牛骨骼肌衍生卫星细胞（MDSC）的影响。我们发现使用Western印迹和免疫荧光实验，在牛MDSCS中上调WDR13。此外，激活和抑制WDR13表达的激活和抑制分别增加和降低细胞分化，表明WDR13促进牛MDSC分化。为了进一步了解WDR13的作用机制，我们在WDR13激活或抑制后检查了PI3K / AKT信号通路的变化。此外，用磷酸阳性激酶3（PI3K）抑制剂，LY294004处理细胞以观察细胞分化。结果表明，WDR13的激活抑制了PI3K / AKT信号通路和增强的细胞分化。这些数据表明WDR13可以通过影响PI3K / AKT信号通路来促进牛MDSC的分化。

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  • 来源
    《Cell biology international.》 |2019年第7期|共10页
  • 作者

    Fu Yuying; Li Shuang; Tong Huili; Li Shufeng; Yan Yunqin;

  • 作者单位

    The Laboratory of Cell and DevelopmentNortheast Agricultural UniversityHarbin 150030 Heilongjiang;

    The Laboratory of Cell and DevelopmentNortheast Agricultural UniversityHarbin 150030 Heilongjiang;

    The Laboratory of Cell and DevelopmentNortheast Agricultural UniversityHarbin 150030 Heilongjiang;

    The Laboratory of Cell and DevelopmentNortheast Agricultural UniversityHarbin 150030 Heilongjiang;

    The Laboratory of Cell and DevelopmentNortheast Agricultural UniversityHarbin 150030 Heilongjiang;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    differentiation; MDSC; MYH3; MYOG; WD repeat domain 13;

    机译：分化;MDSC;MyH3;Myog;WD重复域13;

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