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首页> 外文期刊>Cell biology international. >Image cytometric measurements of diploid, triploid and tetraploid fish erythrocytes in blood smears reflect the true dimensions of live cells.
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Image cytometric measurements of diploid, triploid and tetraploid fish erythrocytes in blood smears reflect the true dimensions of live cells.

机译：血液涂片中二倍体，三倍体和四倍体鱼红细胞的图像细胞仪测量反映了活细胞的真实尺寸。

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摘要

Comparative image cytometry of erythrocytes of diploid and triploid tench Tinca tinca L. and evolutionary tetraploid sterlet Acipenser ruthenus L. was performed on whole live unstained cells, live cells with stained nuclei and on stained fixed whole cells and their nuclei to test if erythrocyte measurements made from blood smears reflect the true dimensions of live cells. Nuclear area and perimeter were the best ploidy level predictors distinguishing accurately among live and fixed diploid, triploid and tetraploid cells, without significant differences between live and fixed cells within a ploidy level. Redundancy analysis revealed insignificant marginal effect of fixation (explained 2.3% of variation, F = 0.804), whereas the effect of ploidy level was highly significant (explained 50.6% of variation, F = 34.874). The erythrocyte measurements of diploid, triploid and tetraploid fish erythrocytes and their nuclei made from blood smears reflect the true dimensions of live cells, and the fixation procedure did not substantially affect their predictive value for ploidy level determination.
机译：二倍体和三倍体Tenca L.和进化四倍体甾体acipenser ruthenus l的对比图像细胞测定术在整个活未染色的细胞上进行，染色核的活细胞和染色固定的整个细胞及其核试验，如果红细胞测量结果从血液涂抹反映活细胞的真实尺寸。核区域和周长是最佳的倍性倍性水平预测因子，在活性和固定的二倍体，三倍体和四倍体细胞中进行准确地区分，没有倍率水平在活体和固定细胞之间的显着差异。冗余分析显示了固定的微不足道的边际效果（解释了2.3％的变异，f = 0.804），而倍率水平的效果非常显着（解释50.6％的变异，F = 34.874）。二倍体，三倍体和四倍体鱼红细胞的红细胞测量和由血液涂片制成的核反映了活细胞的真实尺寸，固定程序基本上没有影响它们的倍频水平测定的预测值。

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