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首页> 外文期刊>Cell biology international. >Adipose tissue‐derived mesenchymal stem cells and keratinocytes co‐culture on gelatin/chitosan/β‐glycerol phosphate nanoscaffold in skin regeneration
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Adipose tissue‐derived mesenchymal stem cells and keratinocytes co‐culture on gelatin/chitosan/β‐glycerol phosphate nanoscaffold in skin regeneration

机译：在皮肤再生中的明胶/壳聚糖/β-甘油磷酸盐纳米支链中脂肪组织衍生的间充质干细胞和角蛋白酶细胞共培养

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Abstract Using cell‐based engineered skin is an emerging strategy for treating difficult‐to‐heal wounds. To date, much endeavor has been devoted to the fabrication of appropriate scaffolds with suitable biomechanical properties to support cell viability and growth in the microenvironment of a wound. The aim of this research was to assess the impact of adipose tissue‐derived mesenchymal stem cells (AD‐MSCs) and keratinocytes on gelatin/chitosan/β‐glycerol phosphate (GCGP) nanoscaffold in full‐thickness excisional skin wound healing of rats. For this purpose, AD‐MSCs and keratinocytes were isolated from rats and GCGP nanoscaffolds were electrospun. Through an in vivo study, the percentage of wound closure was assessed on days 7, 14, and 21 after wound induction. Samples were taken from the wound sites in order to evaluate the density of collagen fibers and vessels at 7 and 14 days. Moreover, sampling was done on days 7 and 14 from wound sites to assess the density of collagen fibers and vessels. The wound closure rate was significantly increased in the keratinocytes‐AD‐MSCs‐scaffold (KMS) group compared with other groups. The expressions of vascular endothelial growth factor, collagen type 1, and CD34 were also significantly higher in the KMS group compared with the other groups. These results suggest that the combination of AD‐MSCs and keratinocytes seeded onto GCGP nanoscaffold provides a promising treatment for wound healing.
机译：摘要采用基于细胞的工程皮肤是一种治疗难以治愈的伤口的新策略。迄今为止，许多努力已经致力于用合适的生物力学性能制造适当的支架，以支持伤口的微环境中的细胞活力和生长。该研究的目的是评估脂肪组织衍生的间充质干细胞（Ad-MSC）和角质形成细胞对大鼠全厚快递皮肤伤口愈合的明胶/壳聚糖/β-甘油磷酸盐（GCGP）纳米键形的影响。为此目的，从大鼠中分离AD-MSC和角蛋白酶，并且GCGP纳米烃型是Electrompul。通过体内研究，在伤口诱导后的第7,14和21天评估伤口闭合的百分比。从伤口部位取样，以便在7和14天内评估胶原纤维和血管的密度。此外，取样是在伤口部位的第7天和14天，以评估胶原纤维和血管的密度。与其他基团相比，角蛋白酶-AD-MSCS-支架（KMS）组中伤口闭合速率显着增加。与其他组相比，KMS组的血管内皮生长因子，胶原1型和CD34的表达也显着较高。这些结果表明，接种在GCGP纳米亚烃形上的Ad-MSC和角蛋白细胞的组合为伤口愈合提供了有希望的处理。

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