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首页> 外文期刊>Cell biology international. >Calycosin stimulates the osteogenic differentiation of rat calvarial osteoblasts by activating the IGF1R/PI3K/Akt signaling pathway
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Calycosin stimulates the osteogenic differentiation of rat calvarial osteoblasts by activating the IGF1R/PI3K/Akt signaling pathway

机译：Calycosin通过激活IGF1R / PI3K / AKT信号通路来刺激大鼠颅骨成骨细胞的骨质发生分化

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摘要

Abstract Calycosin has been reported to have a strong osteogenic activity and a positive correlation with anti‐osteoporosis effects. However, its precise mechanism of action remains unclear. Since insulin‐like growth factor 1 receptor (IGF1R) signaling and phosphatidylinositol 3‐kinase/Akt (PI3K/Akt) signaling have been shown to play a pivotal role in regulating osteogenesis, we hypothesized that the osteogenic activity of calycosin is mediated by these signaling pathways. Rat calvarial osteoblasts (ROBs) were cultured in osteogenic medium containing calycosin with or without GSK1904529A (GSK) or LY294002 (LY) (inhibitors of IGF1R and PI3K, respectively). The effects on cell proliferation, alkaline phosphatase (ALP) activity, calcified nodules, mRNA or protein expression of osteogenic genes [alkaline phosphatase ( Alpl ), collagen type I ( Col1a1 ), runt‐related transcription factor 2 ( Runx2 ), Osterix, and bone morphogenetic protein 2 ( Bmp2 )], and phosphorylation of IGF1R and Akt were examined. The present results showed that calycosin enhanced cell proliferation, ALP activity and Alizarin Red‐S staining in a dose‐dependent manner in the range of 10 ?8 –10 ?6 ?M, while an inhibitory effect was observed at 10 ?5 ?M. Treatment at the optimal concentration (10 ?6 ?M, a physiologically achievable concentration) increased mRNA levels of osteogenic genes and phosphorylation of IGF1R and Akt. Furthermore, treatment with GSK or LY partly reversed the effects of calycosin on ROBs, as indicated by the decreases in calycosin‐induced ALP activity, calcified nodules and osteogenic gene expression. These results suggest that the osteogenic effect of calycosin partly involves the IGF1R/PI3K/Akt signaling pathway.
机译：据报道，摘要的花青素具有强烈的成骨活性和与抗骨质疏松症的正相关性。然而，其精确的行动机制仍不清楚。由于已经显示出胰岛素样生长因子1受体（IGF1R）信号传导和磷脂酰肌醇3-激酶/ AKT（PI3K / AKT）信号传导在调节成骨中发挥枢转作用，我们假设CalycoIn的骨质发生活性由这些信号介导途径。大鼠颅骨成骨细胞（roobs）在含有或没有GSK1904529A（GSK）或LY294002（LY）（分别的IGF1R和PI3K的抑制剂）中培养含有枯牛酸的骨质发生培养基中的培养物。对细胞增殖，碱性磷酸酶（ALP）活性，钙化结节，骨质基因的蛋白质表达的影响[碱性磷酸酶（ALPL），胶原蛋白I型（COL1A1），runt相关转录因子2（RUNX2），Osterix和检查骨形态发生蛋白2（BMP2）]和IGF1R和AKT的磷酸化。目前的结果表明，钙霉素在10〜8-10Ω·6μm的范围内以剂量依赖性方式增强了细胞增殖，ALP活性和茜素红-s染色，同时观察到10？5？m的抑制效果。在最佳浓度（10？6·m，生理学上可实现的浓度）的治疗增加了骨质发生基因的mRNA水平和IGF1R和AKT的磷酸化。此外，用GSK或LY治疗部分逆转了钙霉素对抢夺的影响，如钙霉素诱导的ALP活性，钙化结节和骨质发生基因表达的降低。这些结果表明，枯牛酸的成沸作用部分涉及IGF1R / PI3K / AKT信号通路。

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  • 来源
    《Cell biology international.》 |2019年第3期|共10页
  • 作者

    Fang Yaoyao; Xue Zhiyuan; Zhao Lianggong; Yang Xiuyan; Yang Yafei; Zhou Xianglin; Feng Shilan; Chen Keming;

  • 作者单位

    School of PharmacyLanzhou University199 Donggangxi Road Lanzhou 730000 People's Republic of China;

    School of PharmacyLanzhou University199 Donggangxi Road Lanzhou 730000 People's Republic of China;

    Lanzhou University Second HospitalLanzhou 730000 People's Republic of China;

    School of PharmacyLanzhou University199 Donggangxi Road Lanzhou 730000 People's Republic of China;

    School of PharmacyLanzhou University199 Donggangxi Road Lanzhou 730000 People's Republic of China;

    School of PharmacyLanzhou University199 Donggangxi Road Lanzhou 730000 People's Republic of China;

    School of PharmacyLanzhou University199 Donggangxi Road Lanzhou 730000 People's Republic of China;

    Institute of OrthopaedicsLanzhou General Hospital Lanzhou Command of CPLALanzhou 730050 Gansu;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    calycosin; mineralization; osteoblasts; osteoporosis; PCR; signal transduction;

    机译：calycosin;矿化;成骨细胞;骨质疏松症;PCR;信号转导;

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