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首页> 外文期刊>Cell biology international. >Silver nanoparticles promote osteogenic differentiation of human periodontal ligament fibroblasts by regulating the RhoA–TAZ axis
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Silver nanoparticles promote osteogenic differentiation of human periodontal ligament fibroblasts by regulating the RhoA–TAZ axis

机译：银纳米粒子通过调节RhoA-Taz轴来促进人牙周韧带成纤维细胞的成骨分化

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摘要

Abstract Silver nanoparticles (AgNps) are well established antibacterial agents, which have been reported to promote osteogenesis of stem cells. Ras homolog gene family member A ( RhoA ) and Tafazzin ( TAZ ) have been recently shown to be involved in osteogenic differentiation. The present study aimed to evaluate the effects of AgNps on osteogenic differentiation of human periodontal ligament fibroblasts (HPDLFs), and investigate the underlying mechanisms of AgNps activity. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was employed to determine the safe concentration of AgNps in HPDLFs. Using an alkaline phosphatase assay, Alizarin red S staining and detection of the expression of alkaline phosphatase (ALP), runt‐related transcription factor 2 (RUNX2), osteocalcin (OCN), osterix (OSX), and collagen‐I, we found that AgNps, at a concentration range of 25–100?μM, promoted osteogenic differentiation of HPDLFs in a dose‐dependent manner. We also found that 100?μM AgNps up‐regulated the active RhoA expression level. The results of ALP activity and expression of osteogenic markers showed that the effects of AgNps on osteogenic differentiation were abrogated by a RhoA pathway inhibitor, C3 reagent. Additionally, silencing of TAZ attenuated the AgNps‐induced osteogenic differentiation of HPDLFs, as shown by decreased ALP activity and down‐regulation of osteogenic markers. Interestingly, TAZ knockdown had a very small effect on the activity of RhoA, whereas C3 suppressed the expression of TAZ, indicating that TAZ was a downstream effector of RhoA. In conclusion, the present work demonstrated that AgNps promoted osteogenic differentiation of HPDLFs by activating the RhoA–TAZ axis.
机译：摘要银纳米粒子（AgNP）是完善的抗菌剂，据报道促进干细胞的骨质发生。 Ras Homolog基因家族成员A（RhoA）和Tafazzin（TAZ）已被展示参与骨质发生分化。本研究旨在评估AgNP对人牙周韧带成纤维细胞（HPDLF）的骨质发生分化的影响，并研究AgNPS活性的潜在机制。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑鎓溴（MTT）测定法测定HPDLF中的AgNPS的安全浓度。使用碱性磷酸酶测定，茜素红S染色和检测碱性磷酸酶（ALP）的表达，runt相关转录因子2（RUNX2），骨屈曲（OCN），Ostrox（OSX）和胶原-1，我们发现了AgNP，浓度范围为25-100Ωμm，以剂量依赖性方式促进了HPDLF的成骨分化。我们还发现100？μmagnps上调活性Rhoa表达水平。 ALP活性和成骨标志物的表达结果表明，AGNP对骨质发生分化的影响被RhoA途径抑制剂C3试剂消除。另外，TAZ的沉默减弱了HPDLF的agnps诱导的骨质成骨分化，如通过降低的ALP活性和骨质发生标记的下调所示。有趣的是，TAZ敲低对RhoA的活动产生了非常小的影响，而C3则抑制TAZ的表达，表明TAZ是RHOA的下游效应器。总之，目前的作品证明AgNP通过激活RhOA-Taz轴来促进HPDLF的骨质发生分化。

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  • 来源
    《Cell biology international.》 |2019年第8期|共11页
  • 作者

    Xu Yunhe; Zheng Bowen; He Jia; Cui Zhao; Liu Yi;

  • 作者单位

    Department of Orthodontics School of StomatologyChina Medical University110002 Shenyang People's;

    Department of Orthodontics School of StomatologyChina Medical University110002 Shenyang People's;

    Department of Orthodontics School of StomatologyChina Medical University110002 Shenyang People's;

    Department of General SurgeryChangchun Children's Hospital130061 Changchun People's Republic of;

    Department of Orthodontics School of StomatologyChina Medical University110002 Shenyang People's;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    AgNps; osteogenic differentiation; periodontitis; RhoA; TAZ;

    机译：agnps;成骨分化;牙周炎;rhoa;taz;

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