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首页> 外文期刊>Cell biology international. >Relationship between methylation status of ERCC1 promoter and radiosensitivity in glioma cell lines.
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Relationship between methylation status of ERCC1 promoter and radiosensitivity in glioma cell lines.

机译：胶质瘤细胞系中ERCC1启动子甲基化状态与胶质瘤细胞辐射敏感性的关系。

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Ionizing radiation represents one of the most important therapies for glioma, a lethal primary brain tumor, while radiotherapy remains a challenge for radiation oncologist because of radioresistance. Radiosensitivity of gliomas determines radiotherapy efficacy. Evidence demonstrated that methylation of CpG Island in the promoter region may result in gene silencing. This study was designed to determine the relationship between methylation status of ERCC1 promoter region and radiosensitivity in glioma cell lines. We investigated the expression levels of ERCC1 transcripts and protein in GBM cell lines. Colony forming experiments was used to measure surviving fraction at 2Gy (SF2) in four human glioma cell lines, MGR1, MGR2, SF767 and T98G. Methylation status in the promoter region of ERCC1 in these glioma cell lines was determined by using bisulphate sequencing and MSP analysis. Radiosensitivity was examined to be heterogeneous in these glioma cell lines. There was a statistical difference in the radiosensitivity between glioma cell lines with and without methylation of ERCC1 gene promoter CpG islands. Furthermore, we promoted ERCC1 expression by 5-azacytidine treatment which resulted in the reduction of radiation-induced cell killing in radiosensitive cell lines. Our data indicate that methylation status of ERCC1 is associated with radiosensitivity in glioma cell lines. It could be used as a new biomarker for predicting the radiosensitivity of human gliomas.
机译：电离辐射代表胶质瘤，致死的原发性脑肿瘤最重要的疗法之一，而放射治疗仍然是放射肿瘤学家的挑战，因为辐射侵蚀性。胶质瘤的放射敏感度决定了放射治疗疗效。证据表明，启动子区域中CpG岛的甲基化可能导致基因沉默。本研究旨在确定ERCC1启动子区甲基化状态与胶质瘤细胞系中的放射敏感性之间的关系。我们研究了GBM细胞系中ERCC1转录物和蛋白质的表达水平。菌落形成实验用于在四种人胶质瘤细胞系，MGR1，MGR2，SF767和T98G中测量2GY（SF2）的存活级分。通过使用正硫酸盐测序和MSP分析测定这些胶质瘤细胞系中ERCC1的启动子区域中的甲基化状态。检查辐射敏感性在这些胶质瘤细胞系中是异质的。胶质瘤细胞系与Ercc1基因启动子CpG岛的甲基化的胶质瘤细胞系之间的放射敏感性差异存在统计学差异。此外，我们通过5-氮杂胞苷的处理促进了ERCC1表达，导致在放射敏细胞系中减少辐射诱导的细胞杀伤。我们的数据表明ERCC1的甲基化状态与胶质瘤细胞系中的放射敏感性相关。它可以用作预测人胶质瘤的放射敏感性的新生物标志物。

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