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首页> 外文期刊>Cell biology international. >Sfrp5 expression and secretion in adipocytes are up-regulated during differentiation and are negatively correlated with insulin resistance
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Sfrp5 expression and secretion in adipocytes are up-regulated during differentiation and are negatively correlated with insulin resistance

机译：在分化期间，脂肪细胞中的SFRP5表达和分泌在分化期间上调，与胰岛素抵抗呈负相关

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摘要

We have examined the patterns of Sfrp5 (secreted frizzled-related protein 5) mRNA expression and protein secretion during adipocyte differentiation, and investigated the potential role of Sfrp5 in IR (insulin resistance) in adipocytes. 3T3-L1 pre-adipocytes were induced for differentiation, and RT-PCR (reverse transcription-PCR) and ELISA assays were used to determine Sfrp5 mRNA expression and protein secretion. The results showed that with the differentiation and maturity of pre-adipocytes, transcription and protein secretion of Sfrp5 gradually increased, peaking on the 9th day of differentiation. Sfrp5 mRNA expression in mature adipocytes was decreased by 20, 22 and 32 upon treatment with dexamethasone, insulin and TNF (tumour necrosis factor) respectively, whereas Sfrp5 protein secretion was decreased by 15, 17 and 30%, correspondingly. In contrast, Sfrp5 mRNA expression in mature adipose was increased by 34 and 19% upon treatment with rosiglitazone and metformin respectively, whereas Sfrp5 protein secretion was increased by 10 and 6%, correspondingly. In conclusion, Sfrp5 mRNA expression and protein secretion depend on the differentiation of adipocytes. The dysregulation of Sfrp5 expression and secretion is directly correlated with IR. Up-regulation of Sfrp5 expression and secretion in adipocytes may be one crucial mechanism by which rosiglitazone and metformin improve insulin sensitivity.
机译：我们已经检查了在脂肪细胞分化过程中检测SFRP5（分泌的混浊相关蛋白5）mRNA表达和蛋白质分泌的模式，并研究了SFRP5在Adipocytes中的IR（胰岛素抵抗）的潜在作用。诱导3T3-L1预脂肪细胞进行分化，RT-PCR（逆转录-PCR）和ELISA测定用于确定SFRP5 mRNA表达和蛋白质分泌。结果表明，随着脂肪细胞前脂肪细胞的分化和成熟，SFRP5的转录和蛋白质分泌逐渐增加，在分化的第9天达到峰值。在用地塞米松，胰岛素和TNF（肿瘤坏死因子）处理后，成熟脂肪细胞中的SFRP5 mRNA表达在20,22和32分别下降，而SFRP5蛋白质分泌相应地减少了15,17和30％。相反，成熟脂肪中的SFRP5 mRNA表达分别在用罗格列酮和二甲双胍处理后增加了34和19％，而SFRP5蛋白质分泌相应地增加了10％和6％。总之，SFRP5 mRNA表达和蛋白质分泌取决于脂肪细胞的分化。 SFRP5表达和分泌的缺点与IR直接相关。在脂肪细胞中的SFRP5表达和分泌的上调可能是Rosiglitazone和二甲双胍提高胰岛素敏感性的一个关键机制。

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  • 来源
    《Cell biology international.》 |2012年第9期|共5页
  • 作者

    LvC.; JiangY.; WangH.; ChenB.;

  • 作者单位

    Department of Endocrinology Southwest Hospital Third Military Medical University Chongqing;

    Department of Endocrinology Southwest Hospital Third Military Medical University Chongqing;

    Department of Endocrinology Southwest Hospital Third Military Medical University Chongqing;

    Department of Endocrinology Southwest Hospital Third Military Medical University Chongqing;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    3T3-L1 pre-adipocytes; Adipogenesis; Insulin resistance; Obese; Sfrp5;

    机译：3T3-L1预脂肪细胞;脂肪发生;胰岛素抵抗;肥胖;SFRP5;

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