...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Dental pulp stem cells promote regeneration of damaged neuron cells on the cellular model of Alzheimer's disease
【24h】

Dental pulp stem cells promote regeneration of damaged neuron cells on the cellular model of Alzheimer's disease

机译：牙科纸浆干细胞在阿尔茨海默病的细胞模型上促进受损神经元细胞的再生

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Alzheimer's disease (AD) is an incurable neurodegenerative disease and many types of stem cells have been used inADtherapy with some favorable effects. In this study, we investigated the potential therapeutical effects of human dental pulp stem cells (hDPSCs) on AD cellular model which established by okadaic acid (OA)-induced damage to human neuroblastoma cell line, SH-SY5Y, in vitro for 24 h. After confirmed the AD cellular model, the cells were co-culture with hDPSCs by transwell co-culture system till 24 h for treatment. Then the cytomorphology of the hDPSCs-treated cells were found to restore gradually with re-elongation of retracted dendrites. Meanwhile, Cell Counting Kit-8 assay and Hoechst 33258 staining showed that hDPSCs caused significant increase in the viability and decrease in apoptosis of the model cells, respectively. Observation of DiI labeling also exhibited the prolongation dendrites in hDPSCs-treated cells which were obviously different from the retraction dendrites in AD model cells. Furthermore, specific staining of a-tubulin and F-actin demonstrated that the hDPSCs-treated cells had the morphology of restored neurons, with elongated dendrites, densely arranged microfilaments, and thickened microtubular fibrils. In addition, results from western blotting revealed that phosphorylation at Ser 396 of Tau protein was significantly suppressed by adding of hDPSCs. These results indicate that hDPSCs may promote regeneration of damaged neuron cells in vitro model of AD and may serve as a useful cell source for treatment of AD.
机译：阿尔茨海默病（AD）是一种可治区神经退行性疾病，并且许多类型的干细胞已被使用，具有一些有利的效果。在这项研究中，我们研究了人牙牙髓干细胞（HDPSC）对冈田酸（OA）造成的人神经母细胞瘤细胞系，SH-SY5Y，体外损伤的AD细胞模型对AD细胞模型的潜在治疗作用。在确认AD细胞模型之后，细胞通过Transwell共培养系统与HDPSC共同培养，直至24小时进行治疗。然后发现HDPSC治疗细胞的细胞形态逐渐恢复恢复的树枝状曲线。同时，细胞计数试剂盒-8测定和Hoechst 33258染色表明，HDPSC分别引起了模型细胞的活力和降低的可积累和降低。 DII标记的观察还表现出HDPSCS处理细胞中的延长树突，其与AD模型细胞中的缩回树枝状有明显不同。此外，α-微管蛋白和F-accin的特异性染色证明了HDPSCS处理的细胞具有恢复神经元的形态，具有细长的树枝状细长，致密地布置微丝，以及增稠的微管原纤维。此外，Western印迹结果显示，通过添加HDPSC，Tau蛋白的SER 396的磷酸化显着抑制了HDPSC。这些结果表明，HDPSC可以促进AD的体外模型的受损神经元细胞的再生，并且可以作为用于治疗AD的有用细胞来源。

著录项

  • 来源
    《Cell biology international.》 |2017年第6期|共12页
  • 作者

    Wang Feixiang; Jia Yali; Liu Jiajing; Zhai Jinglei; Cao Ning; Yue Wen; He Huixia; Pei Xuetao;

  • 作者单位

    Chinese Peoples Liberat Army Gen Hosp Dept Stomatol 28 Fuxing Rd Beijing 100853 Peoples R China;

    Acad Mil Med Sci Beijing Inst Transfus Med Stem Cell &

    Regenerat Med Lab 27 Taiping Rd Beijing;

    Acad Mil Med Sci Beijing Inst Transfus Med Stem Cell &

    Regenerat Med Lab 27 Taiping Rd Beijing;

    Acad Mil Med Sci Beijing Inst Transfus Med Stem Cell &

    Regenerat Med Lab 27 Taiping Rd Beijing;

    Acad Mil Med Sci Beijing Inst Transfus Med Stem Cell &

    Regenerat Med Lab 27 Taiping Rd Beijing;

    Acad Mil Med Sci Beijing Inst Transfus Med Stem Cell &

    Regenerat Med Lab 27 Taiping Rd Beijing;

    Chinese Peoples Liberat Army Gen Hosp Dept Stomatol 28 Fuxing Rd Beijing 100853 Peoples R China;

    Acad Mil Med Sci Beijing Inst Transfus Med Stem Cell &

    Regenerat Med Lab 27 Taiping Rd Beijing;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Alzheimer's disease; cell model; dental pulp stem cells; okadaic acid; therapeutical effects;

    机译：阿尔茨海默病;细胞模型;牙科纸浆干细胞;冈田酸;治疗效果;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号