The role of G-protein-coupled membrane estrogen receptor in mouse Leydig cell functionin vivo and in vitro evaluation

Kotula-Balak M.; Pawlicki P.; Milon A.; Tworzydlo W.; Sekula M.; Pacwa A.; Gorowska-Wojtowicz E.; Bilinska B.; Pawlicka B.; Wiater J.; Zarzycka M.; Galas J.

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The role of G-protein-coupled membrane estrogen receptor in mouse Leydig cell functionin vivo and in vitro evaluation

机译：G蛋白偶联膜雌激素受体在小鼠Leydig细胞功能素体内的作用和体外评价

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In this study, G-coupled estrogen receptor (GPER) was inactivated, by treatment with antagonist (G-15), in testes of C57BL/6 mice: immature (3weeks old), mature (3months old) and aged (1.5years old) (50g/kg bw), as well as MA-10 mouse Leydig cells (10nM/24h) alone or in combination with 17-estradiol or antiestrogen (ICI 182,780). In G-15-treated mice, overgrowth of interstitial tissue was found in both mature and aged testes. Depending on age, differences in structure and distribution of various Leydig cell organelles were observed. Concomitantly, modulation of activity of the mitochondria and tubulin microfibers was revealed. Diverse and complex GPER regulation at the mRNA level and protein of estrogen signaling molecules (estrogen receptor and ; ER, ER and cytochrome P450 aromatase; P450arom) in G-15 Leydig cells was found in relation to age and the experimental system utilized (in vivo and in vitro). Changes in expression patterns of ERs and P450arom, as well as steroid secretion, reflected Leydig cell heterogeneity to estrogen regulation throughout male life including cell physiological status.We show, for the first time, GPER with ERs and P450arom work in tandem to maintain Leydig cell architecture and supervise its steroidogenic function by estrogen during male life. Full set of estrogen signaling molecules, with involvement of GPER, is crucial for proper Leydig cell function where each molecule acts in a specific and/or complementary manner. Further understanding of the mechanisms by which GPER controls Leydig cells with special regard to male age, cell of origin and experimental system used is critical for predicting and preventing testis steroidogenic disorders based on perturbations in estrogen signaling.

机译：在该研究中，通过用拮抗剂（G-15）处理，在C57BL / 6小鼠的睾丸中处理G结耦合雌激素受体（GPER）：未成熟（3周龄），成熟（3个月）和老化（1.5年龄））（50g / kg bw），以及单独的MA-10小鼠leydig细胞（10nm / 24h）或与17-雌二醇或抗雌激素组合（ICI 182,780）。在G-15处理的小鼠中，在成熟和老化的睾丸中发现了间质组织的过度生长。根据年龄，观察到各种LEYDIG细胞细胞器的结构和分布的差异。同时，揭示了线粒体和管蛋白微纤维的活性的调节。在雌激素信号分子（雌激素受体和; ER，ER和细胞色素P450芳族芳香酶P450芳族芳香酶P450芳香酶）的不同和复杂的GPER调节。在使用的年龄和实验系统中发现了G-15 Leydig细胞中的（在体内和体外）。 ERS和P450Arom表达模式的变化以及类固醇分泌，反映了整个男性寿命的雌激素调节，包括细胞生理状态。我们首次展示了与串联的ERS和P450AROM的工作，以维持Leydig细胞建筑和雌激素在雌性寿命期间监督其甾体功能。具有GPER累及的全套雌激素信号分子对于适当的LEYDIG细胞功能至关重要，其中每种分子以特定和/或互补的方式作用。进一步了解GPER控制LEYDIG细胞具有特殊考虑男性年龄的机制，所使用的原产地和实验系统的细胞对于预测和预防基于雌激素信号的扰动来说至关重要。

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来源
《Cell and Tissue Research》 |2018年第2期|共24页
作者
Kotula-Balak M.; Pawlicki P.; Milon A.; Tworzydlo W.; Sekula M.; Pacwa A.; Gorowska-Wojtowicz E.; Bilinska B.; Pawlicka B.; Wiater J.; Zarzycka M.; Galas J.;
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作者单位

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Endocrinol Gronostajowa 9 PL-30387 Krakow;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Endocrinol Gronostajowa 9 PL-30387 Krakow;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Endocrinol Gronostajowa 9 PL-30387 Krakow;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Dev Biol &

Invertebrate Morphol;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Endocrinol Gronostajowa 9 PL-30387 Krakow;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Endocrinol Gronostajowa 9 PL-30387 Krakow;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Endocrinol Gronostajowa 9 PL-30387 Krakow;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Endocrinol Gronostajowa 9 PL-30387 Krakow;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Genet &

Evolutionism Gronostajowa 9 PL;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Cell Biol &

Imaging Gronostajowa 9 PL;

Jagiellonian Univ Med Coll Med Biochem Kopernika 7 PL-31034 Krakow Poland;

Jagiellonian Univ Krakow Inst Zool &

Biomed Res Dept Endocrinol Gronostajowa 9 PL-30387 Krakow;

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原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
Leydig cell; G-coupled estrogen receptor; Estrogen receptors; estrogens; Ultrastructure;

机译：Leydig细胞;G型偶联雌激素受体;雌激素受体;雌激素;超微结构;

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1. The role of G-protein-coupled membrane estrogen receptor in mouse Leydig cell functionin vivo and in vitro evaluation [J] . Kotula-Balak M., Pawlicki P., Milon A., Cell and Tissue Research . 2018,第2期

机译：G蛋白偶联膜雌激素受体在小鼠Leydig细胞功能素体内的作用和体外评价
2. The expression of aromatase, estrogen receptor alpha and estrogen receptor beta in mouse Leydig cells in vitro that derived from cryptorchid males [J] . Kotula-Balak M, Gancarczyk M, Sadowska J, European Journal of Histochemistry . 2005,第1期

机译：隐睾雄性小鼠Leydig细胞体外芳香化酶，雌激素受体α和雌激素受体β的表达
3. The expression of aromatase, estrogen receptor a and estrogen receptor b in mouse Leydig cells in vitro that derived from cryptorchid males [J] . M Kotula-Balak, M Gancarczyk, J Sadowska, European Journal of Histochemistry . 2005,第1期

机译：隐睾虫雄性小鼠Leydig细胞体外芳香化酶，雌激素受体a和雌激素受体b的表达
4. The effects of perfluorooctane sulfonate(PFOS) on proliferation of mouse leydig cells in vitro [C] . Zhang De-yong, Xu Xiao-lu, Shen Xiu-ying International Conference on Energy and Environmental Protection . 2013

机译：全氟辛烷磺酸盐（PFOS）对体外小鼠Leydig细胞增殖的影响
5. Design and synthesis of molecular probes to modulate cell membrane permeation: I. Design, synthesis, and biological evaluation of novel chicoric acid analogues as inhibitors of HIV-1 integrase. II. Design, synthesis, and biological evaluation of novel estradiol analogues targeting the membrane estrogen receptor. [D] . Charvat, Trevor Thomas. 2004

机译：设计和合成的分子探针，可调节细胞膜的渗透性：I.设计，合成和生物评价作为抗HIV-1整合酶抑制剂的新型菊苣酸类似物。二。针对膜雌激素受体的新型雌二醇类似物的设计，合成和生物学评估。
6. Telocytes in the mouse testicular interstitium: implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells [O] . Piotr Pawlicki, Anna Hejmej, Agnieszka Milon, -1

机译：小鼠睾丸间质中的卵母细胞：G蛋白偶联雌激素受体（GPER）和雌激素相关受体（ERR）在调节小鼠睾丸间质细胞中的意义
7. The role of G-protein-coupled membrane estrogen receptor in mouse Leydig cell function—in vivo and in vitro evaluation [O] . M. Kotula-Balak, P. Pawlicki, A. Milon, 2018

机译：G蛋白偶联膜雌激素受体在小鼠Leydig细胞功能中的作用 - 体内和体外评价

The role of G-protein-coupled membrane estrogen receptor in mouse Leydig cell functionin vivo and in vitro evaluation

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