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首页> 外文期刊>Cell and Tissue Research >Ontogeny of ramified CD45 cells in chicken embryo and their contribution to bursal secretory dendritic cells
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Ontogeny of ramified CD45 cells in chicken embryo and their contribution to bursal secretory dendritic cells

机译：鸡胚中分枝CD45细胞的组织发生及其对Bursal分泌树突细胞的贡献

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摘要

Embryonic tissues contain highly ramified stellate-shaped cells expressing CD45 and MHC II antigens but their origin and immunophenotype are unknown. Using staged avian embryos and cell-type-specific antibodies, we establish a detailed spatiotemporal ontogeny of cells that express CD45, the earliest marker of hematopoietic stem cells in the chick. CD45 immunostaining marks three distinct embryonic cell populations: round, ramified and amoeboid cells. The round and ramified CD45+ cells appear first in yolk-sac blood islands before the onset of circulation. A subpopulation of round cells co-expresses the thrombocyte-specific CD51/CD61 antigen. Amoeboid cells express macrophage-specific antigens and frequently occur in regions of apoptosis. Ramified cells are distributed uniformly in the embryonic mesenchyme, colonize lymphoid and non-lymphoid organs and later express MHC II. To study the origin of CD45+ cells, 2-day-old chick embryos were ablated from the yolk sac before the establishment of circulation and incubated for 2-5 days. Large numbers of CD45+MHC II+ ramified cells differentiated in the yolk sac. Yolk-sac chimeras were generated by grafting embryos into GFP-expressing de-embryonated yolk sacs. GFP/CD45 co-expressing ramified and amoeboid cells colonized all organ primordia in the donor embryo. We also recombined GFP+ yolk sac with the bursa of Fabricius and found ramified GFP+CD45+ cells in the bursa where they differentiated into dendritic cells. Thus, CD45 cells are first present in the yolk sac during primitive hematopoiesis and then migrate from the extra-embryonic yolk sac to give rise to cells throughout all organ primordia, including dendritic cells in the bursa of Fabricius.
机译：胚胎组织含有高度分枝的星状细胞，表达CD45和MHC II抗原，但它们的起源和免疫表型未知。使用阶段禽胚胎和细胞型抗体，我们建立了表达CD45的细胞的详细时空肌细胞，在小鸡中最早的造血干细胞标记物。 CD45免疫染色标志着三种不同的胚胎细胞群：圆形，分枝和作物细胞。圆形和分枝的CD45 +细胞在循环开始之前首先在卵黄囊血群中出现。圆形细胞的亚群共表现出血小板特异性CD51 / CD61抗原。作用泛细胞表达巨噬细胞特异性抗原，经常发生在细胞凋亡的区域中。分枝细胞均匀地分布在胚胎间能，殖民淋巴结和非淋巴结器官中并发表达MHC II。为了研究CD45 +细胞的起源，在建立循环之前，将2天的雏鸡胚胎从蛋黄SAC中烧蚀并孵育2-5天。大量CD45 + MHC II +分枝细胞在卵黄囊中分化。通过将胚胎移植到表达GFP的去胚卵黄囊中而产生的卵黄囊嵌合体。 GFP / CD45共同表达的分枝和作物细胞在供体胚胎中殖民化所有器官原序。我们还将GFP +蛋黄与Fabricius的Bursa重组，并在囊炎中发现了分枝的GFP + CD45 +细胞，其中它们分化为树突细胞。因此，在原始血液杂物期间，首先在蛋黄囊中存在CD45细胞，然后从胚胎蛋黄囊中迁移到整个器官基金会的所有器官基金会中，包括织物囊中的树突细胞。

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