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首页> 外文期刊>Cell and Tissue Research >Hormone-regulated PKA activity in porcine oviductal epithelial cells
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Hormone-regulated PKA activity in porcine oviductal epithelial cells

机译:猪卵籽上皮细胞中的激素调节的PKA活性

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摘要

The oviduct is a dynamic organ that suffers changes during the oestrous cycle and modulates gamete and embryo physiology. We analyse the possible existence of Protein kinase A (PKA)-dependent hormone-regulated pathways in porcine ampulla and primary cell cultures by 2D-electrophoresis/Western blot using anti-phospho PKA substrate antibodies. Differential phosphorylation was observed for ten proteins that were identified by mass spectrometry. The results were validated for five of the proteins: Annexin A5, Calumenin, Glyoxalase I and II and Enolase I. Immunofluorescence analyses show that Calumenin, Glyoxalase II and Enolase I change their localisation in the oviductal epithelium through the oestrus cycle. The results demonstrate the existence of PKA hormone-regulated pathways in the ampulla epithelium during the oestrus cycle.
机译:输卵管是一种动态器官,其在令人愉快的循环期间发生变化,并调节配子和胚胎生理学。 我们使用抗磷酸PKA底物抗体分析猪Ampulla和主要细胞培养物中蛋白激酶A(PKA)依赖性激素调节途径的可能存在。 观察到通过质谱法鉴定的十种蛋白质的差分磷酸化。 结果验证了五种蛋白质:吞噬蛋白A5,酸甘油蛋白,乙醛酸酶I和II和烯醇酶I.免疫荧光分析表明,Calumenin,乙醛酸酶II和烯醇酶I通过发情循环在输卵管上皮内的定位改变它们的定位。 结果证明了在发情循环期间Ampulla上皮中PKA激素调节途径的存在。

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