...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular immunology >High iodine induces DNA damage in autoimmune thyroiditis partially by inhibiting the DNA repair protein MTH1
【24h】

High iodine induces DNA damage in autoimmune thyroiditis partially by inhibiting the DNA repair protein MTH1

机译：通过抑制DNA修复蛋白MTH1，高碘内诱导自身免疫性甲状腺炎的DNA损伤

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study aims to investigate the level of DNA damage in high iodine (HI)-induced autoimmune thyroiditis (AIT), and to explore the role of DNA repair protein Muff homolog-1 (MTH1) in this process. The levels of pro-inflammatory cytokines tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 were measured using qRT-PCR and ELISA. The apoptosis was evaluated using TUNEL staining. The pathological changes of thyroid tissues were evaluated using hematoxylin and eosin (HE) staining. The DNA damage was assessed by determining the expression of 8-hydroxy-2'deoxyguanosine (8-OHdG; an indicator of oxidative DNA damage) and performing the Comet assay. Our results showed that both the HI-treated NOD.H-2(h4) mice (experimental AIT mice) and the HI-treated mouse thyroid follicular epithelial cells showed enhanced inflammation, apoptosis, and DNA damage level, accompanied by decreased MTH1 expression. Importantly, overexpression of MTH1 effectively abrogated the HI-induced enhancement of inflammation, apoptosis, and DNA damage in mouse thyroid follicular epithelial cells. In conclusion, HI treatment induces DNA damage in AIT, at least in part, by inhibiting the DNA repair protein MTH1.
机译：本研究旨在探讨高碘碘（HI）诱导的自身免疫甲状腺炎（AIT）中DNA损伤的水平，并探讨DNA修复蛋白CUFF同源物 - 1（MTH1）在该过程中的作用。使用QRT-PCR和ELISA测量促炎细胞因子肿瘤坏死因子（TNF） - 白细胞介素（IL）-6和IL-8的水平。使用TUNEL染色评估细胞凋亡。使用苏木精和曙红（HE）染色来评价甲状腺组织的病理变化。通过测定8-羟基-2'deoxyguanote（8-OHDG;氧化DNA损伤的指示剂）和进行彗星测定来评估DNA损伤。我们的研究结果表明，高处理的NOD.H-2（H4）小鼠（实验AIT小鼠）和高处理的小鼠甲状腺上皮细胞显示出增强的炎症，细胞凋亡和DNA损伤水平，伴随着MTH1表达减少。重要的是，MTH1的过度表达有效地废除了小鼠甲状腺卵泡上皮细胞中的炎症，细胞凋亡和DNA损伤的高诱导的增强。总之，通过抑制DNA修复蛋白MTH1，HI治疗至少部分地在AIT中诱导DNA损伤。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号