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首页> 外文期刊>Cellular immunology >Similarities and differences in surface receptor expression by THP-1 monocytes and differentiated macrophages polarized using seven different conditioning regimens
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Similarities and differences in surface receptor expression by THP-1 monocytes and differentiated macrophages polarized using seven different conditioning regimens

机译：用七种不同调理方案对表面受体表达和分化巨噬细胞的相似性和差异

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摘要

Macrophages are key in orchestrating immune responses to micro-environmental stimuli, sensed by a complex set of surface receptors. The human cell line THP-1 has a monocytic phenotype, including the ability to differentiate into macrophages, providing a tractable, standardised surrogate for human monocyte-derived macrophages. Here we assessed the expression of 49 surface markers including Fc, complement, C-type lectin and scavenger receptors; TIMs; Siglecs; and co-stimulatory molecules by flow cytometry on both THP-1 monocytes and macrophages and following macrophage activation with seven standard conditioning/polarizing stimuli. Of the 34 surface markers detected on macrophages, 18 altered expression levels on activation. From these, expression of 9 surface markers were consistently altered by all conditioning regimens, while 9 were specific to individual polarizing stimuli. This study provides a resource for the study of macrophages and highlights that macrophage polarization states share much in common and the differences do not easily fit a simple classification system.
机译：巨噬细胞是通过复杂的表面受体对微环境刺激进行锻造的免疫应答的关键。人体细胞系THP-1具有单核细胞表型，包括分化为巨噬细胞的能力，为人单核细胞衍生的巨噬细胞提供了一种毫噬细胞。在这里，我们评估了49个表面标志物的表达，包括Fc，补体，C型凝集素和清除剂受体;时间; siglecs;通过流式细胞术在THP-1单核细胞和巨噬细胞和巨噬细胞活化中的具有七种标准调理/偏振刺激之后的共刺激分子。在巨噬细胞上检测到的34种表面标记中，激活的18个表达水平改变。由此，通过所有调理方案始终如一地改变9种表面标记的表达，而9对单独的偏振刺激特异。本研究为巨噬细胞的研究提供了一种资源，并突出显示巨噬细胞极化状态共同的共同，并且差异不容易符合简单的分类系统。

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