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首页> 外文期刊>Cellular immunology >Induction of immunostimulatory cytokine genes expression in human PBMCs by a novel semiquinone glucoside derivative (SQGD) isolated from a Bacillus sp. INM-1.
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Induction of immunostimulatory cytokine genes expression in human PBMCs by a novel semiquinone glucoside derivative (SQGD) isolated from a Bacillus sp. INM-1.

机译：用芽孢杆菌分离的新型氨基酮葡糖苷衍生物（SQGD）诱导人PBMCs中的免疫刺激性细胞因子基因表达。 INM-1。

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摘要

In the present study, a semiquinone glucoside derivative (SQGD) isolated from a radioresistant bacterium Bacillus sp. INM-1 was evaluated for its immunostimulatory activities. Human peripheral blood mononuclear cells (PBMCs) were stimulated by different doses (30-90 microg/ml) of SQGD for different time (3-12h) intervals at 37 degrees C, and IL-12p40, IL-23p19, IL-10, RelA and c-Jun gene expression analysis was carried out by qRT-PCR method. SQGD dose dependent cytokines protein expression kinetic analysis was carried out using western blotting. As the results of SQGD (30mug/ml) stimulation for 3h at 37 degrees C, significant induction in IL-12p40, IL-23p19 and RelA gene expression was observed in PBMCs compared to unstimulated control cells. However, no such induction in IL-10 and c-Jun gene expression was observed. Time dependent protein expression study indicated significant increase in IL-12p40, IL-12p35, IL-23p19 and RelA protein expression at 3-6h, which was found decrease at 12h upon SQGD treatment. In contrast, IL-10 protein expression was found to enhance significantly at 12h after SQGD treatment to the PBMCs. SQGD dose dependent study showed approximately similar level of induction in IL-12p40, IL-12p35, IL-23p19 and RelA proteins expression at all tested concentration (30-90 microg/ml) compared to control. However, no significant change in the IL-10 and c-Jun protein expression was observed at any SQGD concentration. SQGD treatment (0.25mg/kgbwt.) was also found to enhance anti-keyhole Limpet Hemocynin (KLH) IgM antibodies significantly in the mice immunized by KLH. Thus, SQGD fraction stimulates cellular immunity by inducing immunostimulatory cytokines and humoral immunity by enhancing IgM antibodies and could be a promising immunostimulant. Further studies related to molecular mechanisms offering immunostimulation is underway, will certainly helpful to unravel its mode of action in the biological system.
机译：在本研究中，从辐射杀菌菌杆菌杆菌SP中分离的半醌葡糖苷衍生物（SQGD）。评估INM-1的免疫刺激活动。人体外周血单核细胞（PBMC）通过不同时间（3-12小时）的不同时间（3-12小时）的SQGD在37℃，IL-12P40，IL-23P19，IL-10，通过QRT-PCR方法进行Rela和C-Jun基因表达分析。 SQGD剂量依赖性细胞因子使用蛋白质印迹进行蛋白表达动力学分析。随着3小时的SQGD（30mug / ml）刺激的结果，与未刺激的对照细胞相比，在PBMC中观察到IL-12P40，IL-23P19和Rela基因表达中的显着诱导。然而，未观察到IL-10和C-JUM基因表达中的这种诱导。时间依赖性蛋白表达研究表明IL-12P40，IL-12P35，IL-23P19和RELA蛋白表达在3-6小时内显着增加，在SQGD处理时发现在12小时内降低。相反，发现IL-10蛋白表达在对PBMC的SQGD处理后12小时内显着增强。 SQGD剂量依赖性研究显示，与对照相比，在所有测试浓度（30-90微孔/ mL）中，IL-12P40，IL-12P35，IL-23P19和Rela蛋白表达中的诱导水平大致相似。然而，在任何SQGD浓度下没有观察到IL-10和C-JUM蛋白表达的显着变化。还发现SQGD治疗（0.25mg / kgBWT。）在通过KLH免疫的小鼠中显着增强抗锁孔颗粒血红素（KLH）IgM抗体。因此，通过增强IgM抗体来诱导免疫刺激细胞因子和体液免疫，可以通过增强免疫刺激剂来刺激细胞免疫刺激细胞免疫。正在进行与分子机制提供免疫刺激的进一步研究，肯定有助于解开其在生物系统中的作用方式。

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