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首页> 外文期刊>Cellular immunology >Induction of immunostimulatory cytokine genes expression in human PBMCs by a novel semiquinone glucoside derivative (SQGD) isolated from a Bacillus sp. INM-1.
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Induction of immunostimulatory cytokine genes expression in human PBMCs by a novel semiquinone glucoside derivative (SQGD) isolated from a Bacillus sp. INM-1.

机译：从芽孢杆菌分离的新型半醌葡糖苷衍生物（SQGD）诱导人PBMC中免疫刺激性细胞因子基因的表达。 INM-1。

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In the present study, a semiquinone glucoside derivative (SQGD) isolated from a radioresistant bacterium Bacillus sp. INM-1 was evaluated for its immunostimulatory activities. Human peripheral blood mononuclear cells (PBMCs) were stimulated by different doses (30-90 microg/ml) of SQGD for different time (3-12h) intervals at 37 degrees C, and IL-12p40, IL-23p19, IL-10, RelA and c-Jun gene expression analysis was carried out by qRT-PCR method. SQGD dose dependent cytokines protein expression kinetic analysis was carried out using western blotting. As the results of SQGD (30mug/ml) stimulation for 3h at 37 degrees C, significant induction in IL-12p40, IL-23p19 and RelA gene expression was observed in PBMCs compared to unstimulated control cells. However, no such induction in IL-10 and c-Jun gene expression was observed. Time dependent protein expression study indicated significant increase in IL-12p40, IL-12p35, IL-23p19 and RelA protein expression at 3-6h, which was found decrease at 12h upon SQGD treatment. In contrast, IL-10 protein expression was found to enhance significantly at 12h after SQGD treatment to the PBMCs. SQGD dose dependent study showed approximately similar level of induction in IL-12p40, IL-12p35, IL-23p19 and RelA proteins expression at all tested concentration (30-90 microg/ml) compared to control. However, no significant change in the IL-10 and c-Jun protein expression was observed at any SQGD concentration. SQGD treatment (0.25mg/kgbwt.) was also found to enhance anti-keyhole Limpet Hemocynin (KLH) IgM antibodies significantly in the mice immunized by KLH. Thus, SQGD fraction stimulates cellular immunity by inducing immunostimulatory cytokines and humoral immunity by enhancing IgM antibodies and could be a promising immunostimulant. Further studies related to molecular mechanisms offering immunostimulation is underway, will certainly helpful to unravel its mode of action in the biological system.
机译：在本研究中，从抗辐射细菌芽孢杆菌中分离出半醌葡萄糖苷衍生物（SQGD）。评价INM-1的免疫刺激活性。在37摄氏度下，通过不同剂量（30-90 microg / ml）的SQGD刺激人外周血单核细胞（PBMC），持续时间不同（3-12h），IL-12p40，IL-23p19，IL-10，通过qRT-PCR方法进行RelA和c-Jun基因表达分析。使用蛋白质印迹进行SQGD剂量依赖性细胞因子蛋白表达动力学分析。作为SQGD（30mug / ml）在37摄氏度下刺激3小时的结果，与未刺激的对照细胞相比，在PBMC中观察到了IL-12p40，IL-23p19和RelA基因表达的显着诱导。然而，没有观察到IL-10和c-Jun基因表达的这种诱导。时间依赖性蛋白表达研究表明，IL-12p40，IL-12p35，IL-23p19和RelA蛋白表达在3-6h显着增加，在SQGD处理后12h降低。相反，发现在对PBMCs进行SQGD处理后12小时，IL-10蛋白表达显着增强。 SQGD剂量依赖性研究显示，与对照相比，在所有测试浓度（30-90微克/毫升）下，IL-12p40，IL-12p35，IL-23p19和RelA蛋白表达的诱导水平大致相似。但是，在任何SQGD浓度下均未观察到IL-10和c-Jun蛋白表达的显着变化。在用KLH免疫的小鼠中，还发现SQGD处理（0.25mg / kgbwt。）可以显着增强抗钥孔血蓝蛋白（KLH）IgM抗体。因此，SQGD级分通过诱导免疫刺激性细胞因子来刺激细胞免疫，并通过增强IgM抗体来增强体液免疫，可能是一种有前途的免疫刺激剂。有关提供免疫刺激的分子机制的进一步研究正在进行中，无疑将有助于阐明其在生物系统中的作用方式。

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