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首页> 外文期刊>Cell cycle >Efficient siRNA delivery by novel PTD-DRBD fusion proteins.
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Efficient siRNA delivery by novel PTD-DRBD fusion proteins.

机译：新型PTD-DRBD融合蛋白的高效siRNA递送。

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摘要

RNA interference (RNAi) was discovered in 1998 and has now spread to become a standard methodology in biology laboratories around the world. RNAi responses are initiated by ectopic delivery of 21-23 nucleotide short interfering dsRNAs (siRNAs) into the cytoplasm of cells. Cytoplasmic siRNAs are then loaded into the RISC RNAi complex which recognizes and degrades target mRNA with an extremely high efficiency (EC_(50)=1 pM). However, due to their size (14,000 Daltons) and strong negative charge, siRNAs have no ability to enter cells on their own and therefore require a delivery agent. Although many materials, such as cationic Lipids and polymers, have been recently developed and old ones enhanced to delivery siRNAs, these delivery agents fail to introduce siRNAs into the entire population of cells and in a non-cytotoxic fashion, especially primary cells and non-adherent cells. Thus, siRNA delivery into cells remains THE rate-limiting step problem for development of RNAi.
机译：RNA干扰（RNAi）于1998年被发现，现已蔓延成为世界各地生物实验室的标准方法。 通过21-23个核苷酸短干扰DSRNA（siRNA）的异位递送来引发RNAi响应，进入细胞的细胞质。 然后将细胞质siRNA加载到RISC RNAi复合物中，识别并降解具有极高效率的靶mRNA（EC_（50）= 1 PM）。 然而，由于它们的尺寸（14,000道尔顿）和强负电荷，SIRNA没有能够自行进入细胞，因此需要送货员。 尽管最近已经开发了许多材料，例如阳离子脂质和聚合物，但是旧的糖果增强到递送siRNA，但这些递送剂未能将siRNA引入整个细胞群和非细胞毒性时装，尤其是主要细胞和非 粘附细胞。 因此，分泌到细胞中的siRNA仍然是RNAi发育的速率限制步骤问题。

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