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首页> 外文期刊>Cell cycle >Mitochondrial regulation of [Ca2+]i oscillations during cell cycle resumption of the second meiosis of oocyte
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Mitochondrial regulation of [Ca2+]i oscillations during cell cycle resumption of the second meiosis of oocyte

机译：[Ca2 +] I振荡的线粒体调节在细胞周期恢复卵母细胞的第二大症期间

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摘要

Oocyte is arrested at metaphase of the second meiosis until fertilization switching on [Ca2+]i oscillations. Oocyte activation inefficiency is the most challenging problem for failed fertilization and embryonic development. Mitochondrial function and intracellular [Ca2+]i oscillations are two critical factors for the oocyte's developmental potential. We aimed to understand the possible correlation between mitochondrial function and [Ca2+]i oscillations in oocytes. To this end, mitochondrial uncoupler CCCP which damages mitochondrial function and two small molecule mitochondrial agonists, L-carnitine (LC) and BGP-15, were used to examine the regulation of [Ca2+]i by mitochondrial functions. With increasing CCCP concentrations, [Ca2+]i oscillations were gradually diminished and high concentrations of CCCP led to oocyte death. LC enhanced mitochondrial membrane potential and [Ca2+]i oscillations and even improved the damage induced by CCCP, however, BGP-15 had no beneficial effect on oocyte activation. We have found that mitochondrial function plays a vital role in the generation of [Ca2+]i oscillations in oocytes, and thus mitochondria may interact with the ER to generate [Ca2+]i oscillations during oocyte activation. Improvement of mitochondrial functions with small molecules can be expected to improve oocyte activation and embryonic development in infertile patients without invasive micromanipulation.
机译：卵母细胞在第二减数分裂中的中期被捕，直到在[CA2 +] I振荡上的受精。卵母细胞活化效率低下是施肥和胚胎发育失败最具挑战性问题。线粒体功能和细胞内[CA2 +] I振荡是卵母细胞发育潜力的两个关键因素。我们旨在了解卵母细胞中线粒体功能和[CA2 +] I振荡之间的可能相关性。为此，使用线粒体功能和两个小分子线粒体激动剂，L-肉碱（LC）和BGP-15的线粒体解耦CCCP用于检查线粒体功能的调节。随着CCCP浓度的增加，[CA2 +] I振荡逐渐减少，高浓度的CCCP导致卵母细胞死亡。 LC增强的线粒体膜电位和[CA2 +] I振荡甚至改善了CCCP诱导的损伤，但是BGP-15对卵母细胞活化没有有益的影响。我们已经发现线粒体函数在卵母细胞中的[Ca2 +] I振荡中发挥着重要作用，因此线粒体可以与ER相互作用以在卵母细胞活化期间产生[Ca2 +] I振荡。可以预期具有小分子的线粒体功能的改善，以改善不侵入的微单据的不孕患者的卵母活化和胚胎发育。

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  • 来源
    《Cell cycle》 |2018年第12期|共16页
  • 作者

    Wang Feng; Yuan Rui-Ying; Li Li; Meng Tie-Gang; Fan Li-Hua; Jing Ying; Zhang Ren-Ren; Li Yuna-Yuan; Liang Qiu-Xia; Dong Feng; Hou Yi; Schatten Heide; Sun Qing-Yuan; Ou Xiang-Hong;

  • 作者单位

    Guangdong Second Prov Gen Hosp Fertil Preservat Lab Reprod Med Ctr Guangzhou Guangdong Peoples;

    Guangdong Second Prov Gen Hosp Fertil Preservat Lab Reprod Med Ctr Guangzhou Guangdong Peoples;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Univ Missouri Dept Vet Pathobiol Columbia MO USA;

    Chinese Acad Sci Inst Zool State Key Lab Stem Cell &

    Reprod Biol Beijing Peoples R China;

    Guangdong Second Prov Gen Hosp Fertil Preservat Lab Reprod Med Ctr Guangzhou Guangdong Peoples;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Oocyte activation; mitochondria; Ca2+i oscillations; mitochondrial membrane potential;

    机译：卵母细胞活化;线粒体;[CA2 +] I振荡;线粒体膜电位;

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