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首页> 外文期刊>Cell cycle >Spontaneous formation of tumorigenic hybrids between human omental adipose-derived stromal cells and endometrial cancer cells increased motility and heterogeneity of cancer cells
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Spontaneous formation of tumorigenic hybrids between human omental adipose-derived stromal cells and endometrial cancer cells increased motility and heterogeneity of cancer cells

机译：人类蛋白脂肪衍生的基质细胞与子宫内膜癌细胞之间的自发形成增加的癌细胞的动力和异质性

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摘要

Recent reports indicate that mesenchymal stem cells (MSCs) can fuse with cancer cells to promote cancer progression. Omental adipose-derived stromal cells (O-ASCs) are similar to MSCs, which could be recruited to the stroma in endometrial cancer. The aim of our study was to investigate whether O-ASCs can fuse with endometrial cancer cells to influence cancer cells biological characteristics. We isolated O-ASCs from patients with endometrial cancer. O-ASCs and endometrial cancer cells were labeled with different fluorescent tags and directly co-cultured in an Opera high-throughput spinning-disk confocal microscopy system to observe the processes involved in the fusion, division and migration of hybrid cells. Immunofluorescence and high-throughput imaging analyzes were performed to evaluate proteins related to epithelial-mesenchymal transition (EMT).We found O-ASCs could spontaneously fuse with endometrial cancer cells, including cytomembrane and nuclear fusion. After fusion, endometrial cancer cells assume an elongated and fibroblast-like appearance that exhibit mesenchymal phenotypes. The hybrid cells proliferated through bipolar and multipolar divisions and exhibited more rapid migratory speeds than were observed in the parental cells (P 0.01), potentially because of their EMT-associated changes, including the down-regulation of E-cadherin and up-regulation of Vimentin. Our results collectively suggest that tumorigenic hybrids spontaneously formed between human O-ASCs and endometrial cancer cells, and that the resulting cells enhanced cancer mobility and heterogeneity by accelerated migration and undergoing multipolar divisions. These data provide a new avenue for investigating the roles of O-ASCs in endometrial cancer.
机译：最近的报道表明，间充质干细胞（MSCs）可以融合癌细胞以促进癌症进展。 Omental脂肪衍生的基质细胞（O-ASCS）类似于MSCs，其可以募集到子宫内膜癌中的基质。我们的研究目的是调查O-ASC是否可以融合子宫内膜癌细胞以影响癌细胞生物学特征。我们从子宫内膜癌患者中孤立O-asc。用不同的荧光标签标记O-ASC和子宫内膜癌细胞，并在液体高通量纺丝盘共杂体显微镜系统中直接共培养，观察杂交细胞融合，分裂和迁移所涉及的过程。进行免疫荧光和高通量成像分析以评估与上皮 - 间充质转换（EMT）相关的蛋白质。我们发现O-ascs可以自发地融合子宫内膜癌细胞，包括细胞膜和核融合。在融合之后，子宫内膜癌细胞呈现细长和成纤维细胞状外观，其表现出间充质表型。通过双极和多极分割增殖的杂交细胞，并且在亲本细胞（P <0.01）中观察到的迁移速度更快，可能是由于其EMT相关的变化，包括e-cadherin的下调和上调 - 平衡调节。我们的结果共同提出，通过加速迁移和遭受多极分割，所得细胞自发地形成的致陀螺杂交物自发地形成。这些数据提供了一种新的途径，用于研究OSCS在子宫内膜癌中的作用。

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  • 来源
    《Cell cycle》 |2019年第3期|共13页
  • 作者

    Li Mingxia; Li Xiaoping; Zhao Lijun; Zhou Jingyi; Cheng Yuan; Xu Bo; Wang Jianliu; Wei Lihui;

  • 作者单位

    Peking Univ Dept Gynecol &

    Obstet Peoples Hosp Beijing Peoples R China;

    Peking Univ Dept Gynecol &

    Obstet Peoples Hosp Beijing Peoples R China;

    Peking Univ Dept Gynecol &

    Obstet Peoples Hosp Beijing Peoples R China;

    Peking Univ Dept Gynecol &

    Obstet Peoples Hosp Beijing Peoples R China;

    Peking Univ Dept Gynecol &

    Obstet Peoples Hosp Beijing Peoples R China;

    Peking Univ Sch Pharmaceut Sci State Key Lab Nat &

    Biomiet Drugs Beijing Peoples R China;

    Peking Univ Dept Gynecol &

    Obstet Peoples Hosp Beijing Peoples R China;

    Peking Univ Dept Gynecol &

    Obstet Peoples Hosp Beijing Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Cell fusion; adipose-derived stromal cells; endometrial cancer; EMT; cancer progression;

    机译：细胞融合;脂肪衍生的基质细胞;子宫内膜癌;EMT;癌症进展;

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