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首页> 外文期刊>Cell cycle >Inhibition of lncRNA Neat1 by catalpol via suppressing transcriptional activity of NF-kappa B attenuates cardiomyocyte apoptosis
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Inhibition of lncRNA Neat1 by catalpol via suppressing transcriptional activity of NF-kappa B attenuates cardiomyocyte apoptosis

机译：通过抑制NF-Kappa B的转录活性抑制NF-Kappa B的转录活性抑制LNCrNA Neat1衰减心肌细胞凋亡

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摘要

Oxidative stress is considered as a major pathogenesis in myocardial damage; however, effective therapies are limited so far. The present study aimed to investigate the in vitro antioxidative mechanism of Catalpol in cardiomyocytes. The results indicated that Catalpol attenuated high glucose (HG)-induced apoptosis in mouse cardiomyocytes via significantly downregulating long noncoding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (Neat1) expression. Furthermore, Catalpol downregulated Neat1 expression and attenuated apoptosis by inhibiting production of intracellular reactive oxygen species (ROS) in HG-treated cardiomyocytes. Moreover, Catalpol also suppressed HG-induced degradation of I kappa B alpha and the nuclear localization of nulear factor-kappa B (NF-kappa B) by decreasing the intracellular ROS levels. Additionally, chromatin immunoprecipitation (ChIP) and dual-luciferase activity assays validated that NF-kappa B bound to Neat1 promoter to activate Neat1 expression. In summary, these results implied that Catalpol protected mouse cardiomyocytes against oxidative injury at least partly through ROS-NF-kappa B-Neat1 axis.
机译：氧化应激被认为是心肌损伤中的主要发病机制;然而，到目前为止，有效的疗法是有限的。本研究旨在探讨Carcyocytes in Catalpol的体外抗氧化机制。结果表明，通过显着下调长的非分量RNA（LNCRNA）核ParaSpechle组装转录1（Neat1）表达，所述催化剂衰减高葡萄糖（Hg） - 在小鼠心肌细胞中诱导小鼠心肌细胞的细胞凋亡。此外，通过抑制HG处理的心肌细胞中的细胞内反应性氧物质（ROS）的产生，Catalpol下调并减弱细胞凋亡。此外，通过降低细胞内ROS水平，Catalpol还抑制了HG诱导的IκBα和氮气因子-Kappa（NF-Kappa B）的核定位。另外，染色质免疫沉淀（芯片）和双荧光素酶活性测定验证了Ne-Kappa B与Neat1启动子结合以激活Neat1表达。总之，这些结果暗示，目录醇保护小鼠心肌细胞，其至少部分地通过ROS-NF-κB-Neat1轴抵抗氧化损伤。

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  • 来源
    《Cell cycle》 |2019年第24期|共10页
  • 作者

    Zou Guoliang; Zhong Weili; Wu Fan; Wang Xiaoxue; Liu Li;

  • 作者单位

    Heilongjiang Univ Chinese Med Affiliated Hosp 1 Dept Cardiovasc Dis Harbin Heilongjiang;

    Heilongjiang Univ Chinese Med Affiliated Hosp 1 Dept Endocrinol Harbin Heilongjiang Peoples R;

    Heilongjiang Univ Chinese Med Affiliated Hosp 1 Dept Cardiovasc Dis Harbin Heilongjiang;

    Heilongjiang Univ Chinese Med Affiliated Hosp 1 Dept Cardiovasc Dis Harbin Heilongjiang;

    Heilongjiang Univ Chinese Med Affiliated Hosp 1 Dept Cardiovasc Dis Harbin Heilongjiang;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Cardiomyocytes; Catalpol; Neat1; NF-kappa B; oxidative stress;

    机译：心肌细胞;Catalpol;Neat1;NF-Kappa B;氧化应激;

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