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首页> 外文期刊>Cytoskeleton >The adhesive disc in the mobilid ciliate Trichodina pediculus: Evidence for centrin-related, calcium-sensitive filaments
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The adhesive disc in the mobilid ciliate Trichodina pediculus: Evidence for centrin-related, calcium-sensitive filaments

机译：运动性纤毛的毛滴虫的黏附盘：与蛋白有关的钙敏感丝的证据

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摘要

The adhesive disc is a highly complex apparatus that allows mobilid ciliates to attach to the tissues of a variety of aquatic invertebrates and vertebrates. The disc comprises concentric rings of rigid skeletal pieces interconnected by filamentous material. This study explored the biochemical properties of the filamentous disc material in the trichodinid Trichodina pediculus. Calcium sensitivity of this material was suggested in vitro by the appearance of transverse cross-striation along bundles of filaments following calcium shock, and complete solubilization of the filamentous material in the presence of EGTA. A 23-kDa immunoanalog of centrins was immunoprecipitated from the EGTA extract. The protein binds calcium as indicated by 45Ca 2+ blot overlay and Ca 2+-induced shifts in electrophoretic mobility. Using Ca 2+/EGTA buffers, we demonstrated a direct relationship between extraction of the filaments and solubilization of the protein. Immunofluorescence and immunoelectron microscopy confirmed that the protein localized to the filamentous disc material and revealed cross-reactivity with the spasmoneme, which is the prototype of ion-sensitive, centrin-like contractile systems in ciliates. The possibility that the filamentous disc material may be a novel example of Ca 2+-sensitive, centrin-based systems found in ciliates is discussed.
机译：粘着盘是高度复杂的设备，其允许活动性纤毛附着到各种水生无脊椎动物和脊椎动物的组织上。盘包括由丝状材料互连的刚性骨架件的同心环。本研究探讨了毛滴虫毛线虫中丝状盘材料的生化特性。通过钙冲击后沿长丝束的横向横条纹的出现以及在EGTA存在下丝状材料的完全溶解表明了这种材料对钙的敏感性。从EGTA提取物中免疫沉淀出23kDa的中心蛋白免疫类似物。如45Ca 2+印迹覆盖和Ca 2+引起的电泳迁移率变化所示，该蛋白质与钙结合。使用Ca 2 + / EGTA缓冲液，我们证明了细丝的提取与蛋白质的溶解之间存在直接的关系。免疫荧光和免疫电子显微镜检查证实该蛋白质定位于丝状盘状材料，并显示出与痉挛性蛋白的交叉反应性，而痉挛性蛋白是纤毛中离子敏感的，类似蛋白的收缩系统的原型。讨论了丝状盘材料可能是纤毛虫中发现的Ca 2+敏感，基于蛋白的系统的新例子的可能性。

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