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首页> 外文期刊>Chemical Reviews >Applications of Infrared Multiple Photon Dissociation (IRMPD) to the Detection of Posttranslational Modifications
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Applications of Infrared Multiple Photon Dissociation (IRMPD) to the Detection of Posttranslational Modifications

机译：红外多光子解离（IRMPD）对后翻版修改检测的应用

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摘要

Infrared multiple photon dissociation (IRMPD) spectroscopy allows for the derivation of the vibrational fingerprint of molecular ions under tandem mass spectrometry (MS/MS) conditions. It provides insight into the nature and localization of posttranslational modifications (PTMs) affecting single amino acids and peptides. IRMPD spectroscopy, which takes advantage of the high sensitivity and resolution of MS/MS, relies on a wavelength specific fragmentation process occurring on resonance with an IR active vibrational mode of the sampled species and is well suited to reveal the presence of a PTM and its impact in the molecular environment. IRMPD spectroscopy is clearly not a proteomics tool. It is rather a valuable source of information for fixed wavelength IRMPD exploited in dissociation protocols of peptides and proteins. Indeed, from the large variety of model PTM containing amino acids and peptides which have been characterized by IRMPD spectroscopy, specific signatures of PTMs such as phosphorylation or sulfonation can be derived. High throughput workflows relying on the selective fragmentation of modified peptides within a complex mixture have thus been proposed. Sequential fragmentations can be observed upon IR activation, which do not only give rise to rich fragmentation patterns but also overcome low mass cutoff limitations in ion trap mass analyzers. Laser-based vibrational spectroscopy of mass-selected ions holding various PTMs is an increasingly expanding field both in the variety of chemical issues coped with and in the technological advancements and implementations.
机译：红外多光子解离（IRMPD）光谱允许在串联质谱（MS / MS）条件下的分子离子的振动指纹衍生。它提供了对影响单氨基酸和肽的后期改性（PTMS）的性质和定位的洞察。利用MS / MS的高灵敏度和分辨率的IRMPD光谱依赖于具有取样物种的IR主动振动模式的共振发生的波长特异性碎片过程，并且非常适合于显示PTM及其存在的存在在分子环境中的影响。 IRMPD光谱显然不是蛋白质组学工具。它是在肽和蛋白质的解离方案中利用的固定波长IRMPD的有价值信息来源。实际上，从含有IRMPD光谱的含氨基酸和肽的含氨基酸和肽的各种型号PTM，可以推导出PTM的特异性差异，例如磷酸化或磺化。因此，提出了依赖于在复杂混合物中改性肽的选择性碎片的高通量工作流。在IR激活时可以观察到序贯碎片，这不仅会产生丰富的碎裂模式，而且还克服了离子阱质量分析仪中的低质量截止限制。持有各种PTMS各种PTMS的基于激光的振动光谱是越来越多的膨胀场，两种化学问题都在与技术进步和实施中的各种化学问题中。

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