...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bulletin of the Chemical Society of Japan >Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-Catalyzed Oxidative Reactions in the Presence of Betaine-Type Metabolite Analogs
【24h】

Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-Catalyzed Oxidative Reactions in the Presence of Betaine-Type Metabolite Analogs

机译：在甜菜碱型代谢物类似物存在下提高辣根过氧化物酶催化氧化反应的发色敏感性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Horseradish peroxidase (HRP), a well-known oxidase, is frequently used in the diagnostic field as a labeling enzyme, where it amplifies the substrate binding signal of antibodies. Though practical for detecting moderate amounts of substrate, there is strong demand for further development of its sensitivity to detect minuscule quantities of biomarkers. Recently, we found that betaine-type cellular metabolite analogs facilitate enzymatic hydrolysis just by dissolving them into the reaction buffer. In the present study, using the analog (2-(N,N,N-tri-n-butylammonium) acetate) and various colorimetric substrates of HRP, we investigated the activation behavior of HRP. As a result, the analog structure- and concentration-dependently facilitated the various HRP-catalyzed oxidative reactions. Interestingly, the analog facilitated not only the reaction rate but also the chromogenic sensitivity. Kinetic and structural analyses revealed that the increased chromogenic sensitivity is related to the enhancement of the conformational flexibility in the substrate binding site in HRP by addition of the analog, which diminishes the binding affinity between HRP and large substrates. The finding serves to create practical applications of the analogs for increased detection sensitivity of HRP-related clinical agents.
机译：辣根过氧化物酶（HRP）是众所周知的氧化酶，通常用于诊断场作为标记酶，其中抗体的底物结合信号。虽然用于检测中等量的底物的实用性，但对其敏感性的敏感性有很强的需求，以检测分数量的生物标志物。最近，我们发现甜菜碱型细胞代谢物类似物促进酶水解，仅通过将它们溶解到反应缓冲液中。在本研究中，使用模拟（2-（N，N，N-三丁基铵）乙酸盐）和HRP的各种比色基底，研究了HRP的活化行为。结果，模拟结构和浓度依赖性地促进了各种HRP催化的氧化反应。有趣的是，模拟不仅促进了反应速率，也促成了发色敏感性。动力学和结构分析表明，通过添加模拟，增加的发色敏性敏感性与HRP中的基材结合位点中的构象柔韧性提高有关，这减少了HRP和大基板之间的结合亲和力。该发现有助于创造模拟的实际应用，以提高与HRP相关的临床药物的检测敏感性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号