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首页> 外文期刊>Acta Horticulturae >Biosafety consideration of different plum pox potyvirus (PPV) genes used for transformation of plants
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Biosafety consideration of different plum pox potyvirus (PPV) genes used for transformation of plants

机译：用于植物转化的不同李子痘病毒（PPV）基因的生物安全性考虑

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摘要

Heterologous encapsidation of potyviruses in transgenic Nicotiana benthamiana, expressing the coat protein gene of an aphid-transmissible PPV isolate (PPV-AT), is outlined. Several new constructs aimed at minimizing the risks connected with the useof viral genes in transgenic Prunus plants are reported. N. benthamiana plants were transformed by Agrobacterium-mediated gene transfer with PPV genes coding for the P1 protein, the cylindrical inclusion body (C1, helicase), the nuclear inclusion body b(NIb, polymerase) and the coat protein (CP) of a non-aphid transmissible isolate. In addition, modified CP genes leading to nontransferable mRNAs in plants were transformed. To facilitate a rapid screening of transformed tissues, the transferred DNA contains, in addition the viral gene, the gus reporter and the nptll antibiotic resistance gene. Transgenic plants expressing the coat protein gene of an aphid transmissible isolate (PPV-AT) were inoculated with different potyviruses. In all cases, various degrees of PPV coat protein were detected in the newly formed virus particles using polyclonal antibodies directed at PPV.
机译：概述了在表达烟草蚜虫可传播PPV分离株（PPV-AT）外壳蛋白基因的转基因烟草本氏烟草中，猪痘病毒的异源衣壳化。报道了几种旨在最小化与在转基因李属植物中使用病毒基因有关的风险的新构建体。通过农杆菌介导的基因转移，将本氏猪笼草植物转化为编码P1蛋白，圆柱形包涵体（C1，解旋酶），核包涵体b（NIb，聚合酶）和外壳蛋白（CP）的PPV基因。非蚜虫可传播隔离株。另外，转化了导致植物中不可转移的mRNA的修饰CP基因。为了促进快速筛选转化的组织，转移的DNA除包含病毒基因外，还包含gus报告基因和nptII抗生素抗性基因。将表达蚜虫可传播分离株（PPV-AT）外壳蛋白基因的转基因植物接种不同的马铃薯病毒。在所有情况下，使用针对PPV的多克隆抗体在新形成的病毒颗粒中检测到不同程度的PPV外壳蛋白。

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