New aspects of virus elimination in fruit trees

Knapp E; Hanzer V; Weiss H; Camara Machado A da; Weiss B; Wang Q; Katinger H; Laimer da Camara Mach

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New aspects of virus elimination in fruit trees

机译：果树病毒清除的新方面

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An in vitro virus elimination programme for Malus and Prunus spp. was carried out using in vitro thermotherapy and meristem tip culture. After the treatments, Malus was tested for apple mosaic ilarvirus (ApMV), apple stem grooving capillovirus (ASGV) and apple chlorotic leaf spot trichovirus (ACLSV), and Prunus for plum pox potyvirus (PPV), prunus necrotic ringspot ilarvirus (PNRV), prune dwarf ilarvirus (PDV), ApMV and ACLSV by ELISA and immuno tissue-printing. PPV, PNRV, ASGV and ACLSV were detectable after 4 years of in vitro culture. PDV was found in an 8-month old Prunus culture. ApMV was lost after 2.5 years of in vitro culture. PPV could be detected in leaves after 20 d of thermotherapy, but not after meristem culture. ASGV was either positive after 33 d of thermotherapy or reduced below the threshold level for reliable serological detection. ASGV titre significantly increased 6 months after thermotherapy and meristem dissection. No ACLSV positives were found after 33 d of thermotherapy, and shoots grown from meristems of main shoots were free of ACLSV 6 months after, whereas shoots from axillary meristems were infected. Nucleic acid-based methods will be used for further evaluation of virus survival during thermotherapy.

机译：一种针对苹果属和李属的体外病毒清除程序。使用体外热疗和分生组织尖端培养进行。治疗后，测试了苹果属苹果花叶病毒（ApMV），苹果茎沟病毒（ASGV）和苹果褪绿叶斑滴虫病毒（ACLSV）以及苹果李对梅花痘病毒（PPV），李属坏死环斑埃拉病毒（PNRV），通过ELISA和免疫组织印记分析梅花矮小病毒（PDV），ApMV和ACLSV。在体外培养4年后可检测到PPV，PNRV，ASGV和ACLSV。 PDV是在8个月大的李属文化中发现的。在2.5年的体外培养后，ApMV丢失。热疗20 d后可在叶片中检测到PPV，而分生组织培养后未检测到。热疗33天后ASGV呈阳性，或降至可靠血清学检测的阈值水平以下。热疗和分生组织剥离后6个月，ASGV滴度显着增加。在热疗33 d后未发现ACLSV阳性，并且从主芽的分生组织生长的芽在6个月后不含ACLSV，而腋生分生组织的芽被感染。基于核酸的方法将用于进一步评估病毒在热疗过程中的存活率。

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来源
《Acta Horticulturae》 |1995年第386期|共10页
作者
Knapp E; Hanzer V; Weiss H; Camara Machado A da; Weiss B; Wang Q; Katinger H; Laimer da Camara Mach;
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正文语种 eng
中图分类园艺;
关键词
plant diseases; plant pathogens; plant disease control; meristems; cultures; DISEASE CONTROL; heat treatment; control; fruit crops; plant pathology; plant viruses; Malus; apple mosaic ilarvirus; apple stem grooving capillovirus; Prunus; plum pox potyvirus; prunus necrotic ringspot ilarvirus; prune dwarf ilarvirus; viruses;

机译：植物病害;植物病原体;植物病害防治;分生组织;培养物;病害防治;热处理;防治措施;水果作物;植物病理学;植物病毒;海棠;苹果花叶病毒;苹果茎槽沟毛细血管病毒;李;李子痘病毒;李子坏死环斑伊拉病毒;修剪矮小伊拉病毒;病毒;

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1. New aspects of virus elimination in fruit trees [J] . Knapp E, Hanzer V, Weiss H, Acta Horticulturae . 1995,第386期

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New aspects of virus elimination in fruit trees

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