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首页> 外文期刊>Acta Horticulturae >CONTINUOUS ETHANOL PRODUCTION BY ZYMOMONAS MOBILIS FROM SAGO STARCH HYDROLYSATE AND NATURAL RUBBER WASTE
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CONTINUOUS ETHANOL PRODUCTION BY ZYMOMONAS MOBILIS FROM SAGO STARCH HYDROLYSATE AND NATURAL RUBBER WASTE

机译：SAGO水解液和天然橡胶废料中发酵单胞菌连续生产乙醇

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摘要

The feasibility of using sago starch hydrolysate (enzyme saccharification) as a carbon source instead of pure glucose and natural rubber serum powder (NRSP) as a nutritional source instead of yeast extract for the medium of ethanol fermentation by Zymomonas mobilis was investigated. The results showed that sago starch hydrolyzed with amylase and NRSP either as it is or hydrolyzed with proteinase have the potential to be used as a fermentation medium resource. It was found that specific glucose uptakerate, specific ethanol production rate and ethanol yield on sago starch hydrolysate with NRSP hydrolysate-Mieki medium (10% initial glucose concentration) are comparable to those values on pure glucose-yeast extract medium. The continuous fermentation in 1 litre jar fermentor with a working volume of 500 ml at pH 5.5, 30 C, was accomplished by pH-stat with separate inflows of alkali and feed medium controlled through a digital set point control (DSC) with two pH set points; a high limit and a low limit. A small difference between the higher pH set point and a lower pH set point and a short control interval time resulted high ethanol productivity, 11.1 g/1 h, with high dilution rate, 0.27 1/h. Higher ethanol productivity for Z. mobilis using the medium consisting of sago starch hydrolysate and rubber industry waste is expected if the measures to release the product inhibition is adopted.
机译：研究了用西米淀粉水解产物（酶糖化）代替纯葡萄糖，天然橡胶血清粉（NRSP）代替酵母提取物作为运动发酵单胞菌乙醇发酵培养基的营养来源的可行性。结果表明，用淀粉酶和NRSP水解的西米淀粉既可以直接使用，也可以用蛋白酶水解，有潜力用作发酵培养基资源。已发现在使用NRSP水解物-Mieki培养基（初始葡萄糖浓度为10％）的西米淀粉水解物上，葡萄糖的比摄取率，乙醇的比产率和乙醇产率与纯葡萄糖-酵母提取物的比值相当。在pH值为5.5、30℃，工作容积为500 ml的1升发酵罐中进行连续发酵是通过pH调节器实现的，其中碱和进料介质分别通过数字设定点控制（DSC）和两个pH设置来控制点上限和下限。高pH设定值与低pH设定点之间的微小差异以及较短的控制间隔时间导致乙醇产量高（11.1 g / 1 h），稀释率高（0.27 1 / h）。如果采取释放产物抑制的措施，则期望使用由西米淀粉水解产物和橡胶工业废料组成的培养基提高运动发酵单胞菌的乙醇生产率。

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