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首页> 外文期刊>Acta Horticulturae >Distribution of myclobutanil fungicide sensitivities among populations of Venturia inaequalis, the causal agent of apple scab, in Ontario.
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Distribution of myclobutanil fungicide sensitivities among populations of Venturia inaequalis, the causal agent of apple scab, in Ontario.

机译：在安大略省苹果黑星病的病原体Venturia inaequalis人群中，对Myclobutanil杀菌剂敏感性的分布。

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摘要

Sensitivity of five populations of V. inaequalis, collected from four geographical locations in Ontario, Canada, to myclobutanil, a demethylation inhibitor fungicide, was investigated. Sensitivities of isolates to fungicide were determined by ED50 values and a sensitivity test based on the relative growth (mean colony diameter on media amended with discriminatory myclobutanil doses per mean colony diameter on unamended media x 100) of mycelial colonies of a baseline population at a single discriminatory dose (0.1 micro g/ml). Both assays were highly correlated. The wild-type population had a baseline sensitivity (mean ED50) of 0.074 micro g/ml of myclobutanil and showed lognormal distributions to myclobutanil. The mean ED50 values were 0.114, 0.074, 0.076, 0.071 and 0.581 micro g/ml of myclobutanil for orchards 1, 2, 3, 4, and 5, respectively. With the exception of the orchard 5, all populations showed lognormal distributions to myclobutanil. Mean isolate sensitivities between baseline population and populations sampled from orchards that have never been or rarely treated with myclobutanil were not significantly different (P=0.20). Isolates from one experimental orchard, orchard 5, that was treated with myclobutanil showed tolerance to the fungicide and had significantly different mean isolate sensitivities than the baseline population (P=0.03). The resistance factor for orchard 5 was 7.8 (mean ED50 of resistant population/mean ED50 of the baseline population), while resistance factors for four other orchards ranged from 0.9 to 1.5 for myclobutanil. Although fungicide resistance to myclobutanil in V. inaequalis was not observed in the commercial orchards, a shift in fungicide sensitivities was observed in an experimental orchard.
机译：研究了从加拿大安大略省的四个地理位置收集的五个种群的V. inaequalis种群对脱甲基化抑制剂杀菌剂myclobutanil的敏感性。分离物对杀菌剂的敏感性由ED50值和敏感性测试确定，该测试基于单个种群的基线菌丝菌落的相对生长（用歧视性霉菌丁胺剂量修正的培养基的平均菌落直径，未经修饰培养基的平均菌落直径×100的平均菌落直径×100）确定。区分剂量（0.1微克/毫升）。两种测定均高度相关。野生型种群的霉菌丁的基线敏感性（平均ED50）为0.074微克/毫升，并显示出与霉菌丁的对数正态分布。对于果园1、2、3、4和5，霉菌丁腈的平均ED50值分别为0.114、0.074、0.076、0.071和0.581微克/毫升。除果园5以外，所有种群对霉菌丁均呈对数正态分布。基线人群与从未或几乎未接受霉菌丁胺处理过的果园采样人群之间的平均分离株敏感性无显着差异（P = 0.20）。用霉菌丁腈处理过的一个实验性果园5的分离株显示出对杀菌剂的耐受性，并且平均分离株的敏感性与基线种群相比有显着差异（P = 0.03）。果园5的抗性因子是7.8（抗性种群的平均ED50 /基线种群的平均ED50），而其他四个果园中的丁菌腈的抗性因子在0.9到1.5之间。尽管在商业性果园中未观察到不等葡萄球菌对霉菌丁腈的抗药性，但在实验果园中却观察到了杀菌剂敏感性的变化。

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