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首页> 外文期刊>Acta Horticulturae >Peach latent mosaic viroid: nucleotide sequence of an Italian isolate, sensitive detection using RT-PCR and geographic distribution
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Peach latent mosaic viroid: nucleotide sequence of an Italian isolate, sensitive detection using RT-PCR and geographic distribution

机译：桃潜在镶嵌类病毒：意大利分离株的核苷酸序列，使用RT-PCR的灵敏检测和地理分布

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摘要

An Italian isolate of peach latent mosaic viroid (PLMVd) was reverse transcribed and amplified from nucleic acid extracts of infected tissue than cloned. Nucleotide sequence analysis of the clone revealed that the viroid is 336-nucleotides long, 95%homologous to the French isolate and contains a conserved sequence of the hammerhead structure which is involved in viroid self-cleavage. DNA primers were utilized for cDNA synthesis and PCR amplification of a full-length viroid DNA product from extracts of PLMVd-infected peach tissue. Amplified PLMVd cDNA hybridized to 32P-labelled PLMVd cRNA probe and was detected from extracts of infected peach fruit, leaf and bark tissues, but not from extracts of uninfected tissue or peach tissue infected with theagent of peach mosaic disease. RT-PCR of peach germplasm from several countries showed that PLMVd is distributed worldwide in peach tissues from Europe, Asia, North America and South America.
机译：从分离的受感染组织的核酸提取物中逆转录并扩增了意大利的桃潜在花叶病毒（PLMVd）分离株。该克隆的核苷酸序列分析表明，该类病毒长336个核苷酸，与法国分离株同源，为95％，并包含锤头结构的保守序列，该序列参与了病毒的自我切割。 DNA引物用于cDNA合成和从感染PLMVd的桃子组织提取物中提取全长病毒DNA产品。扩增的PLMVd cDNA与32P标记的PLMVd cRNA探针杂交，并从感染桃果实，叶片和树皮组织的提取物中检测到，但未从未感染组织或感染桃花叶病的桃组织中检测到。来自多个国家的桃种质的RT-PCR表明，PLMVd分布在欧洲，亚洲，北美和南美的桃子组织中。

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