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首页> 外文期刊>Acta Horticulturae >Advances in asparagus anther culture
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Advances in asparagus anther culture

机译：芦笋花药培养研究进展

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摘要

The effects of triiodobenzoic acid (TIBA) and sucrose concentrations on the embryogenic response of asparagus anther culture were determined. TIBA at 0.05 mg/litre in the induction medium improved the embryogenic response of anthers collected from plants growing under stressed conditions (e.g. insect damage or cold exposure) . TIBA treatment induced embryo formation after a short callusing period. TIBA results were positive in 3 of 5 experiments, and response was associated with plant growth conditions. It is suggested that endogenous hormone levels in the donor plant material and their interactions with TIBA are important to the anther culture response. Low (2%) sucrose concentrations in the induction medium induced embryos that germinated at a frequency of 90%, and the time from culture initiation to plantlet production was 8-10 weeks.
机译：确定了三碘苯甲酸（TIBA）和蔗糖浓度对芦笋花药培养的胚发生反应的影响。在诱导培养基中0.05mg / L的TIBA改善了从在胁迫条件下生长的植物（例如昆虫损伤或冷暴露）中收集的花药的胚发生应答。在短暂的call愈期后，TIBA处理可诱导胚胎形成。在5个实验中的3个中，TIBA结果为阳性，反应与植物生长条件有关。提示供体植物材料中的内源激素水平及其与TIBA的相互作用对花药培养反应很重要。诱导培养基中低（2％）的蔗糖浓度诱导胚芽以90％的频率发芽，从培养开始到幼苗生长的时间为8-10周。

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