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首页> 外文期刊>Acta Horticulturae >Aerial crown-like body formation through nodal segment culture in asparagus
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Aerial crown-like body formation through nodal segment culture in asparagus

机译：通过芦笋节节培养形成空中冠状体

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摘要

An aerial crown-like body is often formed at the node in asparagus. It is histologically similar to the underground crown that forms at the basal portion of the seedling, with numerous crown buds and vascular bundles with no fixed orientation. Formation of the aerial crown-like body was accelerated by culturing nodal shoot segments of young asparagus cv. Mary Washington 500W seedlings in modified MS medium supplemented with 0.5-2 mg ancymidol/litre and 0.1-0.2 M sucrose. In medium supplemented with2 mg ancymidol/litre and 0.1 M sucrose, aerial crown-like body formation and crown bud induction were very frequent. Aerial crown-like body formation was completely suppressed in the presence of 1-10 mg GA3/litre, and GA3 induced lateral shoot development from the lateral buds. Vigorous rooting was accelerated by transferring the nodal segments with an aerial crown-like body to the basal medium. The plantlets obtained were successfully transplanted to soil.
机译：通常在芦笋的结节处形成一个空中的冠状体。它在组织学上类似于在幼苗基部形成的地下树冠，具有许多树冠芽和没有固定方向的维管束。通过培养幼小的芦笋cv的节芽段，加速了空中冠状体的形成。 Mary Washington 500W幼苗，在改良的MS培养基中添加0.5-2 mg兰西多/升和0.1-0.2 M蔗糖。在补充有2 mg恩地米多/升和0.1 M蔗糖的培养基中，空中冠状体形成和冠芽诱导非常频繁。在每升1-10 mg GA3的存在下，空中冠状体的形成被完全抑制，并且GA3诱导了侧芽从侧芽发育。通过将带有空中冠状体的节段转移到基础培养基中，可以加快生根。将获得的幼苗成功移植到土壤中。

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