Increased programmed death-ligand-1 expression in human gastric epithelial cells in Helicobacter pylori infection

WuY.-Y.; LinC.-W.; ChengK.-S.; LinC.; WangY.-M.; LinI.-T.; ChouY.-H.; HsuP.-N.

首页> 外文期刊>Clinical and Experimental Immunology: An Official Journal of the British Society for Immunology >Increased programmed death-ligand-1 expression in human gastric epithelial cells in Helicobacter pylori infection

【24h】

Increased programmed death-ligand-1 expression in human gastric epithelial cells in Helicobacter pylori infection

机译：在幽门螺杆菌感染中的人胃上皮细胞中增加了编程死亡 - 配体-1表达

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Summary B7-H1 [programmed death-ligand-1 (PD-L1)] is a B7-family member that binds to programmed death-1 (PD-1). Recently, deficiency of PD-L1 has been demonstrated to result in accelerated gastric epithelial cell damage in gastritis, and PD-L1 is suggested to play a critical role in regulating T cell homeostasis. Here, we aimed to gain more insight into gastric PD-L1 expression, regulation and function during Helicobacter pylori infection. PD-L1 expression in human gastric epithelial cells was analysed using Western blotting, quantitative polymerase chain reaction and fluorescence activated cell sorter analysis. Furthermore, co-culture experiments of human gastric epithelial cells with primary human T cells or Jurkat T cells were conducted. PD-L1 expression in primary human gastric epithelial cells was strongly enhanced by H. pylori infection and activated T cells, and augmented markedly by further stimulation with interferon-γ or tumour necrosis factor-α. Moreover, PD-L1 expression in gastric epithelial cells significantly induced apoptosis of T cells. Our results indicate that a novel bidirectional interaction between human gastric epithelial cells and lymphocytes modulates PD-L1 expression in human gastric epithelial cells, contributing to the unique immunological properties of the stomach.

机译：摘要B7-H1 [编程死亡 - 配体-1（PD-L1）]是与编程死亡-1（PD-1）结合的B7系列成员。最近，已经证明PD-L1的缺乏导致胃炎中加速的胃液上皮细胞损伤，并且建议PD-L1在调节T细胞稳态中发挥关键作用。在这里，我们旨在在幽门螺杆菌感染期间获得更多地洞察胃PD-L1表达，调节和功能。使用蛋白质印迹，定量聚合酶链反应和荧光活性细胞分选分析分析人胃上皮细胞中的PD-L1表达。此外，进行了用初级人T细胞或Jurkat T细胞进行人胃上皮细胞的共培养实验。通过H.幽门螺杆菌感染和活化的T细胞强大地增强了一次人胃上皮细胞中的PD-L1表达，并通过与干扰素-γ或肿瘤坏死因子-α进一步刺激显着增强。此外，胃上皮细胞中的PD-L1表达显着诱导了T细胞的凋亡。我们的结果表明，人胃上皮细胞和淋巴细胞之间的新型双向相互作用调节人胃上皮细胞中的PD-L1表达，有助于胃的独特免疫特性。

著录项

来源
《Clinical and Experimental Immunology: An Official Journal of the British Society for Immunology》 |2010年第3期|共9页
作者
WuY.-Y.; LinC.-W.; ChengK.-S.; LinC.; WangY.-M.; LinI.-T.; ChouY.-H.; HsuP.-N.;
展开▼
作者单位

Department of Medical Laboratory Science and Biotechnology College of Medicine China Medical;

Department of Medical Laboratory Science and Biotechnology College of Medicine China Medical;

Department of Internal Medicine College of Medicine China Medical University and Hospital;

Department of Medical Laboratory Science and Biotechnology College of Medicine China Medical;

Department of Medical Laboratory Science and Biotechnology College of Medicine China Medical;

Department of Medical Laboratory Science and Biotechnology College of Medicine China Medical;

Department of Medical Laboratory Science and Biotechnology College of Medicine China Medical;

Graduate Institute of Immunology College of Medicine National Taiwan University 1 Jen-Ai Road;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类医学免疫学;
关键词
Apoptosis; B7-H1; Gastric epithelial cell; Helicobacter pylori; PD-1; PD-L1;

机译：细胞凋亡;B7-H1;胃上皮细胞;幽门螺杆菌;PD-1;PD-L1;

相似文献

外文文献
中文文献
专利

1. Increased programmed death-ligand-1 expression in human gastric epithelial cells in Helicobacter pylori infection [J] . WuY.-Y., LinC.-W., ChengK.-S., Clinical and Experimental Immunology: An Official Journal of the British Society for Immunology . 2010,第3期

机译：在幽门螺杆菌感染中的人胃上皮细胞中增加了编程死亡 - 配体-1表达
2. Helicobacter pylori Infection Induces Oxidative Stress and Programmed Cell Death in Human Gastric Epithelial Cells [J] . Song-Ze Ding, Yutaka Minohara, Xue Jun Fan, Infection and immunity . 2007,第8期

机译：幽门螺杆菌感染在人体胃上皮细胞中诱导氧化应激和程序性细胞死亡
3. Helicobacter pylori Infection Is Associated with Increased Expression of Macrophage Migratory Inhibitory Factor--by Epithelial Cells, T Cells, and Macrophages--in Gastric Mucosa. [J] . Xia HH, Lam SK, Huang XR, The Journal of Infectious Diseases . 2004,第2期

机译：幽门螺杆菌感染与胃粘膜中上皮细胞，T细胞和巨噬细胞的巨噬细胞迁移抑制因子表达增加有关。
4. Effect of chronic Helicobacter pylori infection on expression of IKK-β, NF-κB, XIAP in human gastric epithelial cell line [C] . First Zhengzhou International Oncology Congress（ZIOC）- An Update on Diagnosis and Treatment of Solid Tumors, 2005 . 2005

机译：慢性幽门螺杆菌感染对人胃上皮细胞株IKK-β，NF-κB，XIAP表达的影响
5. Gastric epithelial cells act as antigen -presenting cells (APCs): Relevance to Helicobacter pylori infection [D] . Barrera Zamora, Carlos Alberto 2001

机译：胃上皮细胞充当抗原呈递细胞（APC）：与幽门螺杆菌感染的相关性
6. Increased programmed death-ligand-1 expression in human gastric epithelial cells in Helicobacter pylori infection [O] . Y-Y Wu, C-W Lin, K-S Cheng, 2010

机译：幽门螺杆菌感染后人胃上皮细胞程序性死亡配体1表达增加
7. Increased programmed death-ligand-1 expression in human gastric epithelial cells in Helicobacter pylori infection [O] . Wu, Y-Y, Lin, C-W, Cheng, K-S, 2010

机译：幽门螺杆菌感染后人胃上皮细胞程序性死亡配体1表达增加

Increased programmed death-ligand-1 expression in human gastric epithelial cells in Helicobacter pylori infection

摘要

著录项

相似文献

相关主题

期刊订阅