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首页> 外文期刊>Comparative Medicine >Mite Burden and Immunophenotypic Response to Demodex musculi in Swiss Webster, BALB/c, C57BL/6, and NSG Mice
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Mite Burden and Immunophenotypic Response to Demodex musculi in Swiss Webster, BALB/c, C57BL/6, and NSG Mice

机译：对瑞士韦伯斯特，BALB / C，C57BL / 6和NSG小鼠的Demodex Musculi的螨虫和免疫蛋白型反应

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摘要

Detection methods for Demodex musculi were historically unreliable, and testing was rarely performed because its prevalence in laboratory mice was underestimated. Although infestations are unapparent in most mouse strains, D. musculi burdens are higher and clinical signsdetected in various immunodeficient strains. The parasite's influence on the immune system of immunocompetent mice is unknown. We characterized mite burden (immunocompetent and immunodeficient strains) and immunologic changes (immunocompetent strains only) in na?ve Swiss Webster (SW;outbred), C57BL/6NCrl (B6; Th1 responder), BALB/cAnNCrl (BALB/c; Th2 responder) and NOD. Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG; immunodeficient) mice after exposure to Demodex-infested NSG mice. Infested and uninfested age-matched mice of each strain (n= 5) were euthanized 14, 28, 56, and 112 d after exposure. Mite burden was determined through PCR analysis and skin histopathology; B-cell and CD4+ and CD8+ T-cell counts and activation states (CD25 and CD69) were evaluated by using flow cytometry; CBC counts were performed;and serum IgE levels were measured by ELISA. Mite burden and PCR copy number correlated in NSG mice, which had the highest mite burden, but not in immunocompetent strains. Infested immunocompetent animals developed diffuse alopecia by day 112, and both BALB/c and C57BL/6 mice had significantlyincreased IgE levels. These findings aligned with the skewed Th1 or Th2 immunophenotype of each strain. BALB/c mice mounted the most effective host response, resulting in the lowest mite burden of all immunocompetent strains at 112 d after infestation without treatment. Clinically significanthematologic abnormalities were absent and immunophenotype was unaltered in immunocompetent animals. Topical treat- ment with imidacloprid–moxidectin (weekly for 8 wk) was effective at eradicating mites by early as 7 d after treatment. IgE levels decreased substantially in infested BALB/cmice after treatment. These findings demonstrate a need for D. musculi surveillance in mouse colonies, because the infestation may influence the use of infested mice in select studies.
机译：Demodex Musculi的检测方法历史上不可靠，并且很少进行测试，因为它在实验室小鼠中的患病率被低估了。尽管在大多数小鼠菌株中侵扰是不公开的，但D.Musculi负担在各种免疫缺陷菌株中均高且临床。寄生虫对免疫活性小鼠免疫系统的影响是未知的。我们在Na'Ve瑞士韦伯斯特（SW; OUTBRED），C57BL / 6NCRL（B6; TH1响应器），BALB / CANCRL（BALB / C; TH2响应）中，表征了螨虫（仅限免疫活性度和免疫缺陷菌株）和免疫变化（仅限免疫菌株）（仅限免疫菌株）（仅限免疫菌株）（仅限免疫菌株），C57BL / 6NCRL（B6; TH1（BALB / C; TH2响应者） ）和点头。 CG-PRKDCID IL2RGTM1WJL / SZJ（NSG;免疫缺陷）小鼠暴露于Demodex-infested NSG小鼠后。暴露后，每种菌株（n = 5）的侵染和未血液匹配的小鼠在暴露后被安乐死14,28,56和112d。通过PCR分析和皮肤组织病理学确定螨虫负担;通过使用流式细胞仪评估B细胞和CD4 +和CD8 + T细胞计数和活化状态（CD25和CD69）;进行CBC计数;通过ELISA测量血清IgE水平。 NSG小鼠中有螨虫和PCR拷贝数，具有最高的螨虫负担，但不在免疫活性菌株中。侵染的免疫活性动物在第112天开发扩散脱发，BALB / C和C57BL / 6小鼠具有显着的IgE水平。这些发现与每个菌株的偏斜Th1或Th2免疫蛋白型对齐。 BALB / C小鼠安装了最有效的宿主响应，导致侵扰后112天的所有免疫因素菌株的最低螨虫负担而无需治疗。临床上显着的显着性异常不存在，免疫表型在免疫活性动物中未被干扰。用吡虫啉 - 莫克利菌蛋白（每周8周）的局部治疗在治疗后7天恢复螨虫有效。在治疗后，IgE水平在侵染性Balb / Cmice中显着降低。这些发现表明了小鼠菌落中的D. Musculi监测，因为侵扰可能影响在选择研究中使用侵染小鼠的使用。

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  • 来源
    《Comparative Medicine》 |2020年第4期|共13页
  • 作者

    Morris Mariya G; Ricart Arbona Rodolfo J; Daniels Kathleen; Gardner Rui; Easthausen Imaani; Boteler William L; Baseler Gregory P; Pastenkos Gabrielle; Perkins Cheryl L; Henderson Kenneth S; Schietinger Andrea; Lipman Neil S;

  • 作者单位

    Tri-Institutional Training Program in Laboratory Animal Medicine and Science Memorial Sloan Kettering Cancer Center The Rockefeller University and Weill Cornell Medicine;

    Tri-Institutional Training Program in Laboratory Animal Medicine and Science Memorial Sloan Kettering Cancer Center The Rockefeller University and Weill Cornell Medicine;

    Flow Cytometry Core Facility Memorial Sloan Kettering Cancer Center;

    Flow Cytometry Core Facility Memorial Sloan Kettering Cancer Center;

    Division of Biostatistics and Epidemiology Department of Healthcare Policy and Research Weill Cornell Medicine;

    XPressBio;

    XPressBio;

    Center of Comparative Medicine and Pathology Memorial Sloan Kettering Cancer Center and Weill Cornell Medicine;

    Research Animal Diagnostic Services Charles River Laboratories;

    Research Animal Diagnostic Services Charles River Laboratories;

    Program in Immunology Memorial Sloan Kettering Cancer Center;

    Tri-Institutional Training Program in Laboratory Animal Medicine and Science Memorial Sloan Kettering Cancer Center The Rockefeller University and Weill Cornell Medicine;

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  • 正文语种 eng
  • 中图分类 畜牧、动物医学、狩猎、蚕、蜂;
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