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首页> 外文期刊>Comparative Medicine >Allograft Inflammatory Factor 1 as an Immunohistochemical Marker for Macrophages in Multiple Tissues and Laboratory Animal Species
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Allograft Inflammatory Factor 1 as an Immunohistochemical Marker for Macrophages in Multiple Tissues and Laboratory Animal Species

机译：同种异体移植炎症因子1作为多种组织和实验室动物物种中巨噬细胞的免疫组织化学标志物

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Allograft inflammatory factor 1 (AIF1) is a commonly used marker for microglia in the brains of humans and some animal models but has had limited applications elsewhere. We sought to determine whether AIF1 can be used as a macrophage marker across common laboratory animal species and tissues. We studied tissues (that is, spleen, liver, and lung) with defined macrophage populations by using an AIF1 immunostaining technique previously validated in human tissue. Tissues were collected from various mouse strains (n = 20), rat strains (n = 15), pigs (n = 4), ferrets (n = 4), and humans (n = 4, lung only). All samples of liver had scattered immunostaining in interstitial cells, consistent with resident tissue macrophages (Kupffer cells). Spleen samples had cellular immunostaining of macrophages in both the red and white pulp compartments, but the red pulp had more immunostained cellular aggregates and, in some species, increased immunostaining intensity compared with white pulp. In lung, alveolar macrophages had weak to moderate staining, whereas interstitial and perivascular macrophages demonstrated moderate to robust staining. Incidental lesions and tissue changes were detected in some sections, including a tumor, inducible bronchus-associated lymphoid tissue, and inflammatory lesions that demonstrated AIF1 immunostaining of macrophages. Finally, we compared AIF1 immunostaining of alveolar macrophages between a hypertensive rat model (SHR strain) and a normotensive model (WKY strain). SHR lungs had altered intensity and distribution of immunostaining in activated macrophages compared with macrophages of WKY lungs. Overall, AIF1 immunostaining demonstrated reproducible macrophage staining across multiple species and tissue types. Given the increasing breadth of model species used to study human disease, the use of cross-species markers and techniques can reduce some of the inherent variability within translational research.
机译：同种异体移植炎症因子1（AIF1）是人类和一些动物模型中的微胶质植物的常用标志物，但在其他地方有有限的应用。我们试图确定AIF1是否可以用作普通实验室动物物种和组织的巨噬细胞标记。通过使用先前在人体组织中验证的AIF1免疫染色技术，我们将组织（即脾脏，肝脏和肺）与定义的巨噬细胞群进行研究。从各种小鼠菌株（n = 20）中收集组织，大鼠菌株（n = 15），猪（n = 4），菲尔茨（n = 4）和人（仅= 4，肺）。所有肝脏样品在间质细胞中散落过免疫染色，与常规组织巨噬细胞（Kupffer细胞）一致。脾样品在红色和白色纸浆隔室中具有细胞免疫蛋白巨噬细胞，但红色纸浆具有更高的免疫细胞聚集体，并且在一些物种中，与白色纸浆相比增加了免疫染色强度。在肺中，肺泡巨噬细胞对中度染色薄弱，而间隙和血管巨噬细胞表现出中度至鲁棒染色。在某些部分中检测到偶然病变和组织变化，包括肿瘤，诱导的支气管相关的淋巴组织和炎症病变，表明巨噬细胞的AIF1免疫染色。最后，我们比较了高血压大鼠模型（SHR菌株）与正常血压模型（WKY菌株）之间的肺泡巨噬细胞的AIF1免疫染色。与WKY肺的巨噬细胞相比，ShR肺的强度和激活的巨噬细胞中免疫染色的强度和分布。总体而言，AIF1免疫染色涉及多种物种和组织类型的可重复巨噬细胞染色。鉴于用于研究人类疾病的模型物种的增加，使用跨物种标记和技术可以减少翻译研究中的一些固有变化。

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