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首页> 外文期刊>Comparative Medicine >A Comprehensive Protocol for Laparotomy in Swine to Facilitate Ultrasound-guided Injection into the Fetal Intraperitoneal Space
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A Comprehensive Protocol for Laparotomy in Swine to Facilitate Ultrasound-guided Injection into the Fetal Intraperitoneal Space

机译：猪剖腹术的综合方案，促进超声引导注射进入胎儿腹膜内空间

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摘要

Swine are a commonly used animal model for biomedical research. One research application of swine models is the in utero injection of human or pig cells into the fetal liver (FL) or intraperitoneal space. In utero injections can be accomplished through laparotomy procedures in pregnant swine. In this study, we aimed to establish comprehensive laparotomy protocols for ultrasound-guided injections into fetuses. Two pregnant gilts, with a total of 16 fetuses, underwent laparotomy at 41 and 42 d of gestation. During surgery, we attempted to inject half of the fetuses in the FL or intraperitoneal space with saline and titanium wire for radiographic imaging after birth. After the laparotomy and fetal injections, both gilts maintained pregnancy throughout gestation and initiated labor at full term. Of the 16 fetuses present at the time of laparotomy, 12 were liveborn, 2 were stillborn, and the remaining 2 were mummies. A total of 7 fetuses from the 2 litters were known to have been injected with a wire during the surgery. After farrowing, piglets were radiographed, and 6 piglets were identified to have wire within the abdominal space. Livers were dissected, and additional radiographs were obtained. It was determined that one piglet had wire within the liver, whereas the other 5 had wire within the intraperitoneal space. Overall, we describe in-depth laparotomy surgery protocols, ultrasound-guided injection of saline and titanium wire into the FL or intraperitoneal space, postoperative monitoring protocols, and information on radiographic detection of titanium wire after piglet birth. These protocols can be followed by other research groups intending to inject cells of interest into either the intraperitoneal space or FL of fetal piglets.
机译：猪是一种常用的生物医学研究动物模型。猪模型的一项研究应用是中子宫注射人或猪细胞进入胎儿肝（FL）或腹膜内空间。在子宫注射中可以通过妊娠猪的剖腹手术程序来实现。在这项研究中，我们旨在建立综合剖腹手术方案，用于将超声引导注射到胎儿中。两种怀孕的胃肠，共16胎，在41和42 d的妊娠期下进行剖腹手术。在手术过程中，我们试图在出生后用盐水和钛丝注射含有盐水和钛丝的胎儿中的一半。在剖腹手术和胎儿注射后，两种刺激都在妊娠周内保持怀孕，并全部启动劳动力。在曲调术时出现的16个胎儿中，12名均为生身，2次仍然出生，剩下的2个是木乃伊。已知来自2个填充物的7个胎儿在手术期间用电线注射。在分娩后，仔猪射线照相，并鉴定出6个仔猪在腹部空间内有线。解剖肝脏，并获得额外的X线片。确定一个仔猪在肝脏内有线，而另一只猪在腹膜内空间内有线。总体而言，我们描述了深入的剖腹手术协议，超声引导的盐水和钛合线进入FL或腹膜内的空间，术后监测协议以及仔猪初生后钛丝射线检测的信息。这些方案之后可以是任何打算将感兴趣的细胞注入腹腔空间或胎儿仔猪的含量的其他研究组。

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