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首页> 外文期刊>Colloids and Surfaces, B. Biointerfaces >Fabrication of human hair keratin/jellyfish collagen/eggshell-derived hydroxyapatite osteoinductive biocomposite scaffolds for bone tissue engineering: From waste to regenerative medicine products
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Fabrication of human hair keratin/jellyfish collagen/eggshell-derived hydroxyapatite osteoinductive biocomposite scaffolds for bone tissue engineering: From waste to regenerative medicine products

机译：人毛发素的制作/水母胶原蛋白/蛋壳衍生的羟基磷灰石骨诱导生物复合支架，用于骨组织工程：从废物到再生药品

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摘要

In the present study, we aimed at fabricating an osteoinductive biocomposite scaffold using keratin obtained from human hair, jellyfish collagen and eggshell-derived nano-sized spherical hydroxyapatite (nHA) for bone tissue engineering applications. Keratin, collagen and nHA were characterized with the modified Lowry method, free-sulfhydryl groups and hydroxyproline content analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) and thermal gravimetric analysis (TGA) which confirmed the success of the extraction and/or isolation processes. Human adipose mesenchymal stem cells (hAMSCs) were isolated and the cell surface markers were characterized via flow cytometry analysis in addition to multi lineage differentiation capacity. The undifferentiated hAMSCs were highly positive for CD29, CD44, CD73, CD90 and CD105, but were not seen to express hematopoietic cell surface markers such as CD14, CD34 and CD45. The cells were successfully directed towards osteogenic, chondrogenic and adipogenic lineages in vitro. The microarchitecture of the scaffolds and cell attachment were evaluated using scanning electron microscopy (SEM). The cell viability on the scaffolds was assessed by the MTT assay which revealed no evidence of cytotoxicity. The osteogenic differentiation of hAMSCs on the scaffolds was determined histologically using alizarin red S, osteopontin and osteonectin stainings. Early osteogenic differentiation markers of hAMSCs were significantly expressed on the collagen-keratin-nHA scaffolds. In conclusion, it is believed that collagen-keratin-nHA osteoinductive biocomposite scaffolds have the potential of being used in bone tissue engineering. (C) 2017 Elsevier B.V. All rights reserved.
机译：在本研究中，我们旨在使用从人毛，水母胶原蛋白和蛋壳衍生的纳米尺寸球形羟基磷灰石（NHA）获得的角蛋白来制造骨诱导的生物复合支架进行骨组织工程应用。表征角蛋白，胶原蛋白和NHA的特征在于改性的洛数法，自由砜基团和羟脯氨酸含量分析，十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS-PAGE），减弱总反射率 - 傅里叶变换红外光谱（ATR-FTIR）和热量重量分析（TGA）证实提取和/或隔离过程的成功。分离人脂肪糖间充质干细胞（HAMSCs），除了多谱系分化容量之外，通过流式细胞术分析表征细胞表面标志物。未分化的HAMSCs对CD29，CD44，CD73，CD90和CD105具有高度阳性，但未见到表达造血细胞表面标记，例如CD14，CD34和CD45。将细胞成功地朝向骨质发生，软骨生成和脂肪生成谱系进行体外。使用扫描电子显微镜（SEM）评估支架和细胞附着的微体系结构。通过MTT测定评估支架上的细胞活力，其显示没有细胞毒性的证据。使用茜素红S，骨膨胀素蛋白和骨膜染色蛋白染色，在组织学上测定HAMSCS对支架上的骨质发生分化。在胶原蛋白 - 角蛋白 - NHA支架上显着表达了HAMSCs的早期成骨分化标志物。总之，据信胶原蛋白 - 角蛋白-NHA骨诱导性生物复合支架具有用于骨组织工程的潜力。 （c）2017 Elsevier B.v.保留所有权利。

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