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首页> 外文期刊>Clinical and experimental pharmacology & physiology >Regulation of thyroid sodium‐iodide symporter in different stages of goiter: Possible involvement of reactive oxygen species
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Regulation of thyroid sodium‐iodide symporter in different stages of goiter: Possible involvement of reactive oxygen species

机译：甲状腺素不同阶段的甲状腺钠 - 碘化物交响者调节：反应性氧

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Summary Na + /I ? symporter ( NIS ) transports iodide into thyrocytes, a fundamental step for thyroid hormone biosynthesis. Our aim was to evaluate NIS regulation in different status of goitrogenesis and its underlying mechanisms. Wistar rats were treated with methimazole ( MMI ) for 5 and 21?days, to achieve different status of goiter. We then evaluated the effect of MMI removal for 1?day (R1d), after 5 (R1d‐5d) or 21 (R1d‐21d) days of MMI treatment. MMI increased thyroid weight, iodide uptake and in vitro TPO activity in a time‐dependent way. Although MMI removal evoked a rapid normalization of TPO activity in R1d‐5d, it was still high in R1d‐21d. On the other hand, iodide uptake was rapidly down‐regulated in R1d‐21d, but not in R1d‐5d, suggesting that the increased TPO activity in R1d‐21d led to increased intraglandular organified iodine (I‐X), which is known to inhibit iodide uptake. Since TGF β has been shown to mediate some effects of I‐X, we evaluated TGF β and TGF β receptor mRNA levels, which were increased in R1d‐21d. Moreover, it has been demonstrated that TGF β stimulates NOX 4. Accordingly, our data revealed increased NOX 4 expression and H 2 O 2 generation in R1d‐21d. Finally, we evaluated the effect of H 2 O 2 on NIS function and mRNA levels in PCCL 3 thyroid cell line, which were reduced. Thus, the present study suggests that there is a relationship between the size of the goiter and NIS regulation and that the mechanism might involve I‐X, TGF β, NOX 4 and increased ROS production.
机译：摘要na + /我？ Symporter（NIS）将碘化物转化为甲状腺细胞，是甲状腺激素生物合成的基本步骤。我们的目标是评估不同地位的NIS规则及其潜在机制。 Wistar大鼠用甲巯基（MMI）处理5和21℃，以达到甲状腺肿的不同状态。然后，我们评估MMI去除1？日（R1D），5（R1D-5D）或21（R1D-21D）天数的MMI处理的影响。 MMI以时间依赖的方式增加甲状腺重量，碘化物吸收和体外TPO活性。虽然MMI去除诱发R1D-5D中TPO活性的快速标准化，但在R1D-21D中仍然很高。另一方面，在R1D-21D中碘化物摄取迅速下调，但不在R1D-5D中，表明R1D-21D中的TPO活性增加导致含有众所周知的内部有组织的碘（I-X）的TPO活性抑制碘化物摄取。由于TGFβ已经显示为介导I-X的一些效果，我们评估了TGFβ和TGFβ受体mRNA水平，其在R1D-21D中增加。此外，已经证明TGFβ刺激NOx 4.因此，我们的数据显示出增加的NOx 4表达和H 2 O 2在R1D-21D中产生。最后，我们评估了H 2 O 2对PCC13甲状腺细胞系中的NIS功能和mRNA水平的影响，降低了。因此，本研究表明，甲状腺肿和NIS调节的大小之间存在关系，并且该机制可能涉及I-X，TGFβ，NOx 4并增加ROS生产。

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