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首页> 外文期刊>Acta Oto-Laryngologica >Age-related change in the expression of NKCC1 in the cochlear lateral wall of C57BL/6J mice
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Age-related change in the expression of NKCC1 in the cochlear lateral wall of C57BL/6J mice

机译：C57BL / 6J小鼠耳蜗侧壁NKCC1表达的年龄相关变化

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Conclusions: Na+-K+-2Cl-co-transporter isoform 1 (NKCC1) mRNA and protein decrease with increasing age in the cochlear lateral wall of C57BL/6J (C57) mice. The down-regulation of NKCC1 may influence the K+transport efficiency and the homeostasis of ion transport cells, and cause the irreversible damage of cochlear cells in old C57 mice. Our results indicate that NKCC1 may play an important role in the pathogenesis of age-related hearing loss (AHL). Objectives: The aim of the present study was to investigate the relationship between the functional expression of NKCC1 transporter and the etiology of AHL. Methods: C57 mice were used and randomly divided into four groups according to age (4 weeks, 14 weeks, 26 weeks, and 52 weeks). Immunofluorescence technique, quantitative real-time PCR, and western blot were applied to detect the expression of NKCC1 in the cochlear lateral wall of C57 mice at the various ages. Results: In all four groups, the expression of NKCC1 was observed in the stria vascularis and type II fibrocytes of the spiral ligament. Also, the expression of NKCC1 appeared to decrease with age at both the transcriptional level and the protein level.
机译：结论：C57BL / 6J（C57）小鼠的耳蜗侧壁Na + -K + -2Cl-co-转运蛋白亚型1（NKCC1）mRNA和蛋白随年龄的增长而降低。 NKCC1的下调可能影响K +转运效率和离子转运细胞的稳态，并导致老龄C57小鼠的耳蜗细胞不可逆转地受损。我们的结果表明，NKCC1可能在与年龄有关的听力损失（AHL）的发病机理中起重要作用。目的：本研究旨在探讨NKCC1转运蛋白的功能性表达与AHL病因之间的关系。方法：使用C57小鼠，根据年龄（4周，14周，26周和52周）随机分为四组。免疫荧光技术，定量实时荧光定量PCR和Western blot技术检测了不同年龄C57小鼠耳蜗侧壁NKCC1的表达。结果：在所有四个组中，在螺旋韧带的血管纹状体和II型纤维细胞中均观察到NKCC1的表达。同样，NKCC1的表达在转录水平和蛋白质水平上都随着年龄的增长而降低。

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