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首页> 外文期刊>Clinical and experimental allergy : >Higher immunoglobulin E antibody levels to recombinant Fel d 1 in cat-allergic children with asthma compared with rhinoconjunctivitis.
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Higher immunoglobulin E antibody levels to recombinant Fel d 1 in cat-allergic children with asthma compared with rhinoconjunctivitis.

机译：哮喘患者患有哮喘患者的哮喘患儿的重组FEC D 1重组患者的抗体水平较高。

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BACKGROUND: Current diagnosis of allergy and asthma to cat is confirmed using cat dander extract (CDE). We have previously engineered a recombinant major cat allergen, rFel d 1, with properties identical to the natural molecule. OBJECTIVE: The aim of the study was to evaluate IgE and IgG4 antibodies to rFel d 1 among sera from cat-allergic children and adults suffering from asthma and/or rhinoconjunctivitis (RC) in populations from Sweden and Austria. METHODS: Cat-allergic children and adults from Sweden (n=27 and 31, respectively) and Austria (n=41 and 41) with RC and/or asthma were selected. Sera were tested for IgE and IgG4 antibodies to CDE and rFel d 1 by CAP, and IgE to rFel d 1 by ELISA. Healthy subjects and non-cat-allergic patients (n=75) were included as controls. RESULTS: There was a high correlation between IgE responses to rFel d 1 and CDE among the 140 patients (r(s)=0.85, P<0.001); however, measured levels to rFel d 1 were on average 30% higher (P<0.0001). Ninety-eight percent of patients and none of the controls showed IgE to rFel d 1 and there was a threefold increased risk of asthma for half of the children with the highest IgE levels [odds ratio 3.23; 95% confidence interval (CI), 1.19-8.79] by ELISA. IgE responses to rFel d 1 among children with asthma were higher (median 19.4 kU/L) compared with children with RC (median 6.6 kU/L, P<0.05) and adults with asthma (median 3.0 kU/L, P<0.01). Furthermore, children with asthma displayed higher IgG4 levels than the asthmatic adults. CONCLUSION: A single recombinant molecule, rFel d 1, is at least as sensitive for in vitro diagnostics of cat allergy as the current extract-based test. Elevated IgE antibody levels to Fel d 1 are suggested to be a risk factor for asthma in cat-allergic children.
机译：背景：使用猫黄素提取物（CDE）确认了对猫过敏和哮喘的目前诊断。我们以前设计了重组主要猫过敏原，RFEL D 1，属性与天然分子相同。目的：该研究的目的是评估IgE和IgG4抗体在患有瑞典和奥地利人口患有哮喘和/或鼻咽炎（RC）的血清中的血清中的RFEL D 1。方法：选定来自瑞典的猫过敏性儿童和成人（分别为瑞典（n = 27和31）和奥地利（n = 41和41），具有RC和/或哮喘。通过ELISA对CDE和IgG4抗体进行IgE和IgG4抗体测试IgE和IgG4抗体，并通过ELISA对RFEL D 1进行测试。将健康的受试者和非猫过敏患者（n = 75）作为对照。结果：140名患者中对RFEL D 1和CDE之间的IgE反应之间存在高相关（R（S）= 0.85，P <0.001）;然而，测量水平为RFEL D 1平均高度较高（P <0.0001）。患者的百分之九十八分之一患者向rfel d 1显示IgE，并且有三倍的哮喘风险增加了患有最高的IgE水平[赔率比3.23; ELISA的95％置信区间（CI），1.19-8.79]。与哮喘患儿的患儿对RFEL D 1的IgE反应较高（中位数19.4 ku / L）与患有RC的儿童（中位数6.6Ku / L，P <0.05）和具有哮喘的成人（中位数3.0ku / L，P <0.01） 。此外，具有哮喘的儿童显示出比哮喘成人更高的IgG4水平。结论：单一重组分子，RFEL D1，至少与基于提取物的基于提取物的猫过敏的体外诊断一样敏感。升高的IgE抗体水平与Fel D 1的抗体水平被认为是猫过敏性儿童哮喘的危险因素。

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