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首页> 外文期刊>Clinical and experimental allergy : >Concomitant sensitization to legumin, Fag e 2 and Fag e 5 predicts buckwheat allergy
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Concomitant sensitization to legumin, Fag e 2 and Fag e 5 predicts buckwheat allergy

机译：伴随着乳糜，FAG E 2和FAG E 5预测了荞麦过敏的敏感性

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Summary Background Buckwheat ( Fagopyrum esculentum ) has become increasingly popular as a healthy food in Europe. However, for sensitized individuals, consumption can cause anaphylactic reactions. The aim of this study was to identify individual well‐characterized buckwheat allergens for component‐resolved diagnosis. Methods Patients were selected by positive skin prick test to buckwheat and divided into two groups: (1) sensitized to buckwheat without clinical symptoms and (2) buckwheat allergy. Buckwheat proteins were extracted from raw buckwheat seeds, purified applying a combination of protein precipitation and chromatographic methods, and analyzed by IgE immunoblotting and ELISA . Results Buckwheat‐allergic patients had a significantly larger median skin prick test weal diameter for buckwheat than the sensitized group and the positive control. Also, IgE immunoblotting clearly showed a distinct pattern in sera from allergic patients when compared to sensitized individuals. Several IgE‐reactive proteins were purified from crude buckwheat extract, namely legumin (Fag?e?1 plus its large subunit), Fag?e?2 (2S albumin), and newly identified Fag e 5 (vicilin‐like) as well as hevein‐like antimicrobial peptides, designated Fag?e?4. All four allergens showed superior diagnostic precision compared to extract‐based Immuno CAP with high sensitivity as well as high specificity. Conclusions Patients with clinical symptoms clearly show a distinct allergen recognition pattern. We characterized a buckwheat vicilin‐like protein as a new relevant marker allergen, designated Fag e 5. Additionally, another new allergen, Fag?e?4, potentially important for cross‐reactivity to latex was added to the allergen panel of buckwheat. Further, our data show that the full‐length legumin comprising both, large and small subunit should be applied for component‐resolved diagnosis. Our data indicate that concomitant sensitization to legumin, Fag e 2 and Fag e 5, predicts buckwheat allergy.
机译：发明内容背景荞麦（Fagopyrum Esculentum）越来越受到欧洲健康的食物。然而，对于致敏的个体，消费会导致过敏反应。本研究的目的是鉴定个体特征的荞麦过敏原，用于组分分辨诊断。方法将患者用阳性皮肤刺测试选择，以荞麦，分为两组：（1）敏感到荞麦，没有临床症状和（2）荞麦过敏。从原料荞麦种子中提取荞麦蛋白，纯化蛋白质沉淀和色谱方法的组合，并通过IgE免疫印迹和ELISA分析。结果荞麦 - 过敏性患者具有比敏化组和阳性对照的荞麦中位数较大的中位皮肤刺刺试验。此外，与敏化个体相比，IgE免疫印迹清楚地显示出来自过敏患者的血清中的明显图案。从粗荞麦提取物中纯化几种IgE反应性蛋白质，即肉豆蔻（FAG？e≤1加上其大亚基），FAG？2（2S白蛋白），以及新发现的FAG E 5（类似Vicilin）以及蜂巢状抗菌肽，指定FAG？E？4。与基于提取的免疫帽相比，所有四个过敏原表明均匀的诊断精度，具有高灵敏度和高特异性。结论临床症状的患者显然显示出明显的过敏原识别模式。我们将荞麦类蛋白样蛋白质为新的相关标记过敏原，指定的FAG E 5.此外，另一种新的过敏原，FAG？4，对荞麦的过敏原小组中加入了对乳胶的交叉反应性的可能性重要。此外，我们的数据表明，包括兼容大小亚单位的全长豆科术应申请组件分辨诊断。我们的数据表明，伴随着豆科，FAG E 2和FAG E 5的敏感性，预测了荞麦过敏。

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    Geiselhart S.; Nagl C.; Dubiela P.; Pedersen A. C.; Bublin M.; Radauer C.; Bindslev‐Jensen C.; Hoffmann‐Sommergruber K.; Mortz C. G.;

  • 作者单位

    Department of Pathophysiology and Allergy ResearchMedical University of ViennaVienna Austria;

    Department of Pathophysiology and Allergy ResearchMedical University of ViennaVienna Austria;

    Department of Pathophysiology and Allergy ResearchMedical University of ViennaVienna Austria;

    Department of Dermatology and Allergy CenterOdense University HospitalOdense Denmark;

    Department of Pathophysiology and Allergy ResearchMedical University of ViennaVienna Austria;

    Department of Pathophysiology and Allergy ResearchMedical University of ViennaVienna Austria;

    Department of Dermatology and Allergy CenterOdense University HospitalOdense Denmark;

    Department of Pathophysiology and Allergy ResearchMedical University of ViennaVienna Austria;

    Department of Dermatology and Allergy CenterOdense University HospitalOdense Denmark;

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  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    allergens; buckwheat; component‐resolved diagnosis; food allergy; IgE;

    机译：过敏原;荞麦;组分分辨诊断;食物过敏;IgE;

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