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Phenotypic and functional alterations of regulatory B cell subsets in adult allergic asthma patients

机译：成人过敏性哮喘患者中调节B细胞亚群的表型和功能改变

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Abstract Background IL‐10‐producing regulatory B cells (Bregs) are widely ascribed immune regulatory functions. However, Breg subsets in human asthma have not been fully investigated. Objective We studied Breg subsets in adult allergic asthma patients by assessing two major parameters, frequency and IL‐10 expression. We then investigated factors that affect these two parameters in patients. Methods Peripheral blood mononuclear cells (PBMCs) of adult allergic asthma patients (N?=?26) and non‐asthmatic controls (N?=?28) were used to assess the frequency of five subsets of transitional B cells (TBs), three subsets of CD24 high CD27 + B cells and B1 cells. In addition to clinical data, IL‐10 expression by individual Breg subsets was assessed by flow cytometry. Results Asthma patients had decreases of CD5 + and CD1d + CD5 + , but an increase of CD27 + TBs which was significant in patients with moderate asthma (60??FEV1??80). Regardless of asthma severity, there was no significant alteration in the frequencies of 6 other Breg subsets tested. However, we found that oral corticosteroid (OCS) significantly affected the frequency of Bregs in Breg subset‐specific manners. OCS decreased CD5 + and CD1d + CD5 + TBs, but increased CD27 + TBs and CD10 + CD24 high CD27 + cells. Furthermore, OCS decreased IL‐10 expression by CD27 + TBs, all 3 CD24 high CD27 + B cell subsets (CD5 + , CD10 + and CD1d + ) and B1 cells. OCS‐mediated inhibition of IL‐10 expression was not observed in the other Breg subsets tested. Conclusion & Clinical Relevance Alterations in the frequency of Bregs and their ability to express IL‐10 are Breg subset‐specific. OCS treatment significantly affects the frequency as well as their ability to express IL‐10 in Breg subset‐specific manners.
机译：摘要背景IL-10产生的调节B细胞（BREGS）是广泛归因于免疫调节功能。然而，人类哮喘中的Breg子集没有得到完全调查。目的我们通过评估两个主要参数，频率和IL-10表达来研究成人过敏性哮喘患者的Breg子集。然后我们调查了影响患者这两个参数的因素。方法使用成年过敏性哮喘患者的外周血单核细胞（PBMCS）（n？=Δ26）和非哮喘对照（n？=Δ28）来评估过渡性B细胞（TBS）的五个亚组的频率CD24高CD27 + B细胞和B1细胞的子集。除了临床资料外，通过流式细胞术评估单个BREG亚群的IL-10表达。结果哮喘患者减少了CD5 +和CD1D + CD5 +，但增加了CD27 + TBS，其在中度哮喘患者中具有显着的（60〜ΔFEV1？<80）。无论哮喘严重程度如何，在测试的其他6个伯格子集的频率下都没有显着的改变。然而，我们发现口腔皮质类固醇（OCS）显着影响了BREG子集合式的贫困频率。 OCS降低了CD5 +和CD1D + CD5 + TBS，但CD27 + TBS和CD10 + CD24高CD27 +细胞增加。此外，OCS通过CD27 + TBS的IL-10表达减少，所有3个CD24高CD27 + B细胞亚群（CD5 +，CD10 +和CD1D +）和B1细胞。在测试的其他Breg子集中未观察到OCS介导的IL-10表达的抑制。结论＆amp; Bregs频率的临床相关性改变及其表达IL-10的能力是特异性的。 OCS治疗显着影响频率以及在BREG子集合式中表达IL-10的能力。

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