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Emergence of SHV-2a producing enterobacter cloacae in hungary.

机译:匈牙利出现产生SHV-2a阴沟肠杆菌的现象。

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摘要

We report an investigation on a collection of multidrug-resistant Enterobacter cloacae isolates from a Hungarian neonatal intensive care unit. All the isolates (n=142) were examined by antimicrobial susceptibility testing and ERIC-PCR. The seven ESBL positive isolates (derived from six patients) made up a separated group with regard to their patterns by antimicrobial susceptibility testing and ERIC-PCR and were further tested by class-1-integron PCR and plasmid electrophoresis. The ESBL isolates were found indistinguishable in each of these laboratory tests, one genetic clone were revealed in the background of ESBL cases by PFGE. The ESBL positive isolates were proven to harbour a approximately 62 Md plasmid and two class-1 integrons (0.9 kb, 1.875 kb). With respect to their clinical relatedness and our laboratory findings there was a small outbreak caused by the ESBL clone. PCR-sequencing were performed on the outbreak strain and has revealed a blaSHV gene that encodes for an SHV-2a type ESBL enzyme. This is the first description of SHV-2a positive E. cloacae strains isolated in Hungary.
机译:我们报告了对匈牙利新生儿重症监护病房多重耐药性阴沟肠杆菌分离株的调查。通过抗菌药敏试验和ERIC-PCR检查了所有分离物(n = 142)。通过抗菌药敏试验和ERIC-PCR将7种ESBL阳性分离株(来自6例患者)从其模式上分为一个单独的组,并通过1类整合子PCR和质粒电泳进一步进行了检测。在每个实验室测试中均未发现ESBL分离株,PFGE在ESBL病例背景中发现了一个基因克隆。事实证明,ESBL阳性分离株具有约62 Md质粒和两个1类整合素(0.9 kb,1.875 kb)。关于它们的临床相关性和我们的实验室发现,有一个由ESBL克隆引起的小规模暴发。对暴发菌株进行了PCR测序,结果显示了一个blaSHV基因,该基因编码SHV-2a型ESBL酶。这是在匈牙利分离的SHV-2a阴沟肠杆菌阳性菌株的首次描述。

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