• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences >Isolation and characterization of two types of actinophages infecting Streptomyces scabies MR13.
【24h】

Isolation and characterization of two types of actinophages infecting Streptomyces scabies MR13.

机译：感染链霉菌sc疮MR13的两种类型的噬菌体的分离和鉴定。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two types of actinophages, phi S and phi L, were isolated from soil samples by using Streptomyces scabies MR13, a potato scab pathogen, as an indicator strain. The phages were partially characterized according to their physicochemical properties, plaques and particles morphology and their host-range. The host-range of these phages was narrow for phi S and wide for phi L. The adsorption rate constants of the phi S and phi L were 3.44 x 10(-9) and 3.18 x 10(-9) ml/min, and their burst sizes were 1.61 and 3.75 virions, respectively. One-step growth indicated that phi S and phi L have a latent period of 30 min followed by a rise period of 30 min. The temperate character of these phages was tested in other isolates of Streptomyces. Four of the phages (phi SS3, phi SS12, phi SS13 and phi SS17) were identified as temperate phages, since they were able to lysogenize SS3, SS12, SS13 and SS17. phi SS3, phi SS12 and phi SS13 were homoimmune, and they were heteroimmune with respect to phi SS17. The restriction barriers of lysogenic isolates (SS12, SS13 and SS17) interfered with the blockage of plaques formation by phages (phi SS12, phi SS13 or phi SS17) propagated on them, about 75% of lysogenic isolates had restriction systems. The exposure of the lysogenic isolates (SS12, SS13 and SS17) to UV-irradiation prevented the possible restriction barriers of these isolates, and these barriers could be overcome.
机译：使用链霉菌sc疮MR13（一种马铃薯sc疮病原体）作为指示菌株，从土壤样品中分离出两种类型的放线噬菌体phi S和phiL。根据噬菌体的理化性质，噬菌斑和颗粒形态以及寄主范围对它们进行部分表征。这些噬菌体的宿主范围对于phi S较窄，对于phi L较宽。phi S和phi L的吸附速率常数分别为3.44 x 10（-9）和3.18 x 10（-9）ml / min，并且它们的爆发大小分别为1.61和3.75病毒粒子。一步生长表明phi S和phi L的潜伏期为30分钟，其后为上升期30分钟。在链霉菌的其他分离物中测试了这些噬菌体的温带特性。四个噬菌体（phi SS3，phi SS12，phi SS13和phi SS17）被鉴定为温和噬菌体，因为它们能够裂解SS3，SS12，SS13和SS17。 phi SS3，phi SS12和phi SS13是纯免疫的，相对于phi SS17是杂免疫的。溶原性分离物（SS12，SS13和SS17）的限制性障碍物干扰了在其上繁殖的噬菌体（phi SS12，phi SS13或phi SS17）对噬菌斑形成的阻碍，其中约75％的溶原性分离物具有限制性系统。溶原性分离株（SS12，SS13和SS17）暴露于紫外线辐射下，阻止了这些分离株的可能限制屏障，这些屏障可以克服。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号