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首页> 外文期刊>Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences >Genetics of sulphate assimilation in Schizosaccharomyces pombe (a short review).
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Genetics of sulphate assimilation in Schizosaccharomyces pombe (a short review).

机译：粟酒裂殖酵母中硫酸盐同化的遗传学（简短回顾）。

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摘要

Sulphur plays an important role in yeasts, especially in the biosynthesis of methionine and cysteine. The inorganic sulphur source, sulphate, is taken up by the cells via the sulphate-permease(s). After its transport, it is activated and subsequently reduced to sulphide or serves as a donor for sulphurylation reactions. Selenate anion (SeO4(2-)), which has the same metabolic pathway as sulphate, is toxic for the cells of Schizosaccharomyces pombe. We isolated selenate resistant mutants which cannot utilize sulphate, therefore they need organic sulphur source for growth. One of the selenate resistant mutants was successively transformed with S. pombe genomic libraries and the gene complementing the selenate resistance was identified as that of coding for the ATP-sulphurylase enzyme.
机译：硫在酵母中起着重要作用，尤其是在蛋氨酸和半胱氨酸的生物合成中。无机硫源硫酸盐通过硫酸盐渗透酶被细胞吸收。在运输之后，它被活化并随后还原成硫化物或用作硫磺化反应的供体。硒酸根阴离子（SeO4（2-））与硫酸根具有相同的代谢途径，对粟酒裂殖酵母的细胞有毒。我们分离了不能利用硫酸盐的抗硒酸盐突变体，因此它们需要有机硫源来生长。用S. pombe基因组文库连续转化了一个抗硒酸盐的突变体，鉴定出与硒酸盐抗性互补的基因是编码ATP-硫酸酯酶的基因。

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