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首页> 外文期刊>Biotechnologie, Agronomie Societe et Environnement >Comparison of first stages of somatic embryogenesis in Baillonella toxisperma and Vitellaria paradoxa
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Comparison of first stages of somatic embryogenesis in Baillonella toxisperma and Vitellaria paradoxa

机译：产气荚膜芽孢杆菌和悖卵Vitellaria paradoxa体细胞胚发生第一阶段的比较

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In order to initiate the in vitro propagation of Baillonella toxisperma and Vitellaria paradoxa, a comparative study of somatic embryogenesis was studied on these two Sapotaceae using leaf fragment from plants under production. A culture medium of Murashige and Skoog half strength (MS/2) supplemented with 0.6% agar and 4.5% sucrose was used as the basic medium. 0.5 mg.l(-1) of 6-benzylaminopurine (BAP) and 0.5 to 5 mg.l(-1) of 2.4-dichlorophenoxyacetic acid (2.4-D) were added to the basic medium to study the process of callogenesis and the formation of the somatic embryo on the callus. The BAP/2.4-D combination significantly affected callogenesis in both species. During 28 days of culture, the most reactive combinations are 0.5/0.5 in B. toxisperma which allowed 75.2% of the explants to form callus, and 0.5/3 in V paradoxa which permitted to obtain up to 87.3% callogenesis. At 3 mg.1(-1), 2.4-D permitted the differentiation of somatic embryos in 92.7% of callus in B. toxisperma with an average of 38 embryos per callus. On the contrary in V paradoxa, the most reaction is observed with the combination of 0.5/2 BAP/2.4-D which permitted to obtain 62.1% of embryonic callus in 97 days of culture with an average of 27 embryos per callus. The histological study of embryonic callus showed that the development of the differentiated somatic embryos occurs through the same stages in both species; notably the nodular stage, the division of nodular cells stage and the bipolar embryos stage, thus the respective percentages obtained are 77.2% and 66.8% in B. toxisperma and V paradoxa. Though this work opens probably new way of propagation of these two species, the transformation of the bipolar embryos into viable plants remains important phase to study.
机译：为了启动毒双孢杆菌和Vitellaria paradoxa的体外繁殖，利用正在生产的植物的叶片片段，对这两个Sapotaceae进行了体细胞胚发生的比较研究。使用补充有0.6％琼脂和4.5％蔗糖的Murashige和Skoog半强度（MS / 2）的培养基作为基本培养基。将0.5 mg.l（-1）的6-苄基氨基嘌呤（BAP）和0.5至5 mg.l（-1）的2.4-二氯苯氧基乙酸（2.4-D）添加到基本培养基中，以研究of发生的过程和愈伤组织上体细胞胚的形成。 BAP / 2.4-D组合显着影响两个物种的愈伤组织。在培养的28天中，活性最高的组合是：在B. toxisperma中为0.5 / 0.5，使75.2％的外植体形成愈伤组织，在V. paradoxa中为0.5 / 3，允许获得87.3％的愈伤组织。剂量为3 mg.1（-1）时，2.4-D可使豆蔻芽胞杆菌愈伤组织中92.7％的体细胞胚分化，每个愈伤组织平均有38个胚。相反，在V悖论中，用0.5 / 2 BAP / 2.4-D的组合观察到最多的反应，其允许在97天的培养中获得62.1％的胚愈伤组织，每个愈伤组织平均有27个胚。胚胎愈伤组织的组织学研究表明，在两个物种中，分化的体细胞胚的发育都经历相同的阶段。尤其是结节期，结节细胞分裂期和双极胚胎期，因此在豆蔻芽孢杆菌和V型悖论中分别获得的百分比分别为77.2％和66.8％。尽管这项工作可能为这两个物种的繁殖开辟了新的途径，但双极胚胎向有生命的植物的转化仍然是重要的研究阶段。

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